中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 新陆早33号高效遗传转化体系研究 新疆农业科学 2013,50 (6) 981-987
Position: Home > Articles > Study on Protocol of Efficiency Genetic Transformation in Xinluzao 33(Gossypium hirsutum L.) Xinjiang Agricultural Sciences 2013,50 (6) 981-987

新陆早33号高效遗传转化体系研究

作  者：
李鹏飞;朱华国;程文翰;王凡龙;张新宇;孙杰
单  位：
石河子大学农学院/新疆生产建设兵团绿洲生态农业重点实验室
关键词：
棉花;抗性胚性愈伤组织;体细胞胚胎发生;再生植株
摘  要：
【目的】以新疆北疆棉区主栽品种新陆早33号为材料,研究不同处理条件对转基因抗性胚性愈伤组织增殖、胚状体分化以及植株再生的影响,为新疆棉花遗传转化奠定基础。【方法】以新陆早33号下胚轴为外植体,利用农杆菌介导法转化抗草甘膦基因EPSPS,通过优化培养条件以提高抗性胚性愈伤组织获得完整再生植株的能力。【结果】在胚性愈伤组织增殖阶段,培养基中KNO_3浓度为3.8g/L,继代周期为11 d有助于胚性愈伤组织快速增殖和保持良好生长状态;将凝固剂Phytagel浓度提高到4.0g/L能明显促进胚状体分化;在子叶胚生根阶段以Phytagel为凝固剂同时加入1.0 g/L的活性炭有助于壮根、减少褐化和提高正常植株比例。【结论】通过对培养条件的优化,缩短了胚状体发生的周期,并获得了大量再生植株,建立了新陆早33号的高效遗传转化体系。
译  名：
Study on Protocol of Efficiency Genetic Transformation in Xinluzao 33(Gossypium hirsutum L.)
作  者：
LI Peng-fei,ZHU Hua-guo,CHENG Wen-han,WANG Fan-long,ZHANG Xin-yu,SUN Jie (College of Agronomy/Key Laboratory of Oasis Eco-agriculture of Xinjiang Production and Construction Corps,Shihezi University,Shihezi Xinjiang 832003,China)
关键词：
cotton;;resistant embryogenic callus;;somatic embryogenesis;;regenerated plants
摘  要：
[Objective]In order to carry out the transgenic protocol in cotton,the effect of different treatments on resistant embryogenic callus proliferation,somatic embryogenesis and regeneration of upland cotton(Gossypium hirsutum L.) Xinluzao 33 was studied.[Method]Hypocotyls of Xinluzao 33 were taken as explants,and glyphosate - resistant gene EPSPS was transformed by Agrobacterium - mediated transformation method.Somatic embryogenesis capacity and cotyledonary embryos culture methods were optimized.[Result] During the period of embryogenic callus proliferation,when the concentration of the culture medium was 3.8 g/L KNO_3,11 days subculture cycle were suitable for fast proliferation and keeping better state.Phytagel in MSB medium supplemented with 4.0 g/L obviously promoted embryoid differentiation,and with 1.0 g/L activated carbon facilitated root development,reduced browning and improved the ratio of normal regenerated plant.[Conclusion]The cycle of regeneration via somatic embryogenesis was shortened and a large number of regenerated plants were obtained.The efficient genetic transformation protocol for Xinluzao 33 was constructed.

相似文章

导出引用
计量
文章访问数: 16
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46