Position: Home > Articles > Immunocapture-Universal Primer PCR Detection of Salmonella sp. in Foods
FOOD SCIENCE
2009,30
(12)
233-236
免疫捕捉-通用引物PCR检测食品中沙门氏菌
作 者：
张体银;黄晓蓉;郑晶;邵碧英;黄嫦娇;陈彬;林杰;汤敏英
单 位：
福建出入境检验检疫局
关键词：
免疫捕捉;通用引物;PCR;沙门氏菌;食品
摘 要：
利用免疫吸附富集结合经典PCR技术建立免疫捕捉通用引物PCR(IC-UPPCR)检测食品中沙门氏菌。采用细菌16SrRNA基因保守区设计特异性引物,建立通用引物PCR技术是可行的,该IC-UPPCR检测沙门氏菌的灵敏度最低,能检测到2×102CFU/ml,检测沙门氏菌属和非沙门氏菌属标准株的特异性为100%,无假阳性和假阴性出现。结果表明该方法具有简单、快速、特异性好和敏感性高等特点,并可满足大批样品沙门氏菌筛选检测的要求,适用于食品卫生监管、商品检验检疫以及临床诊断等领域。
译 名：
Immunocapture-Universal Primer PCR Detection of Salmonella sp. in Foods
作 者：
ZHANG Ti-yin,HUANG Xiao-rong,ZHENG Jing,SHAO Bi-ying,HUANG Chang-jiao,CHEN Bin,LIN Jie,TANG Min-ying(Fujian Entry-Excit Inspection and Quarantine Bureau,Fuzhou 350001,China)
关键词：
immunocapture;universal primer;PCR;Salmonella sp.;foods
摘 要：
An immunocapture-universal primer polymerase chain reaction (PCR) (IC-UPPCR) method was developed by combining the floride ion-selective electrode method with classical PCR assay for detection of Salmonella sp.in food and its sensitivity and specificity were investigated.It was feasible to establish a universal primer PCR technology was by designing specific primers from conserved regions of bacterial 16S ribosomal RNA genes.The sensitivity of the IC-UPPCR to detect Salmonella sp.was 2 × 102 CFU/ml and the specificity was 100% without any false negative or positive cases.The results demonstrate that the IC-UPPCR is a simple,rapid,highly sensitive and specific method for the detection of Salmonella sp.in the foods and suitable for detection of Salmonella sp.in a large number of food samples.
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