Position: Home > Articles > Determination of the Content of Flavonoid in Different Parts of Cynanchum cathayense from Xinjiang
Journal of Tarim University
2010,22
(4)
75-77
新疆产羊角子草不同部位中总黄酮的含量测定
作 者：
孟庆艳;孔星芸;田龙;方玉
单 位：
塔里木大学生命科学学院;塔里木盆地生物资源保护利用兵团重点实验室
关键词：
羊角子草;芦丁;紫外-可见分光光度法;鹅绒藤属
摘 要：
目的建立羊角子草中总黄酮含量的测定方法,并分析该类成分在羊角子草不同部位的分布情况。方法用超声法提取羊角子草中总黄酮;采用紫外-可见分光光度法于508 nm处测定总黄酮的含量。结果总黄酮在22.78~113.88μg.mL-1浓度范围内与吸光度有良好的线性关系(r=0.999 9),羊角子草中总黄酮的含量为:根0.28%,茎0.77%,叶1.60%,花1.06%,果实0.49%,平均回收率为98.62%,RSD为1.58%(n=9)。结论该方法简便、快速、准确,可用于羊角子草中总黄酮含量的测定。
译 名：
Determination of the Content of Flavonoid in Different Parts of Cynanchum cathayense from Xinjiang
作 者：
Meng Qingyan1,2 Kong Xingyun1 Tian Long2 Fang Yu2(1 Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin,Xinjiang Production & Construction Group,Alar,Xinjiang 843300)(2 College of Life Science,Tarim University,Alar,Xinjiang 843300)
关键词：
Cynanchum cathayense;Flavonoid;UV;Cynanchum
摘 要：
Objective:To establish an method for the determination of flavonoid in Cynanchum cathayense,and analyze the content of flavonoid in different parts of Cynanchum cathayense.Methods:The flavonoid was extracted by the ultrasonic method.The content of flavonoid was detected by UV at wavelength of 508 nm.Results:The calibration curve was linear with the concentration ranges of 22.78~113.88 μg ·mL-1(r=0.999 9).The contents in the root,bark,leaf,flower and fruit are separately 0.28%,0.77%,1.60%,1.06 %and 0.49%.Average recovery was 98.62 %,with RSD of 1.58 %(n=9).Conclusions:this way is handy、celerity、nicety.It can be used to discriminate and dominate the mass of Cynanchum cathayens.
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