Study on the Cultivation of Lactobacillus of Adaptive Acclimation to the Juice of Potato Cane

ZHANG Xiong-jie;LU Peng-fei;CHEN Hao;LI Zhi-qiang;LIU Quan-guo;Innner Mongolia Horizon Oasis Research Center of Characteristic Biologic Resource;Inner Mongolia Agriculture University;Innner Mongolia Greed Seed industry of Potato Co.,Ltd;

potato cane;;Lactobacillus plantarum;;Glycosides alkaloids;;silage

In the experiment,the plant lactobacillus which have high acid production was transferred into a group of MRS liquid nutrient medium for the continuous transfer and cultivation,In this group of 5 liquid medium the juice of potato cane was the ladder of increasing state.Then,the freeze drying of the strains after continuous cultivation was carried out.Further,after thawing activation of the domesticated bacteria and not domesticated bacteria synchronously,They were inoculated to the liquid nutrient medium which included the juice of potato cane cultivate 48 h.After the training the potato cane solid particles and liquid parts in the medium was each separated.The contents of the Glycosides alkaloids in the solid and liquid parts were determined respectively by colorimetric method;p H values and total bacterial count were determined in the liquid parts.The results showed that in the cultivation system of the domesticated bacteria,the total bacterial cell number was significantly higher than that of the control group;p H value was significantly lower than that of the control group;Glycosides alkaloids content in the potato cane solid particles was significantly lower than that of the control group;Glycosides alkaloids in the liquid were significantly higher than those in the control group.