中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 葡萄糖和木糖共发酵生产L-乳酸的培养基和培养条件响应面优化 食品科学 2011,32 (9) 140-145
Position: Home > Articles > Optimization of Co-fermentation of Glucose and Xylose for L-Lactic Acid Production Using Response Surface Methodology FOOD SCIENCE 2011,32 (9) 140-145

葡萄糖和木糖共发酵生产L-乳酸的培养基和培养条件响应面优化

作  者：
潘丽军;庞锐;吴学凤;罗水忠;姜绍通
单  位：
合肥工业大学生物与食品工程学院
关键词：
葡萄糖;木糖;L-乳酸;响应面法;优化
摘  要：
为提高米根霉利用葡萄糖、木糖共发酵产L-乳酸的产量,在单因素试验基础上,采用响应面法进行培养基和培养条件优化的试验方案设计。利用Design Expert软件对其结果进行二次回归分析,获得的最佳产酸条件为:葡萄糖100g/L、木糖50g/L、(NH4)2SO4 3.0g/L、KH2PO4 0.3g/L、摇床转速180r/min、温度32℃、接种量12%、装液量50mL。该条件下摇瓶发酵72h,L-乳酸产量为119.216g/L,转化率为79.48%。
译  名：
Optimization of Co-fermentation of Glucose and Xylose for L-Lactic Acid Production Using Response Surface Methodology
作  者：
PAN Li-jun,PANG Rui,WU Xue-feng,LUO Shui-zhong,JIANG Shao-tong(School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China)
关键词：
glucose;xylose;L-lactic acid;response surface method;optimization
摘  要：
The medium composition and culture conditions for L-lactic acid production via the co-fermentation of glucose and xylose with Rhizopus oryzae were optimized by response surface methodology.A 7-factor,3-level central composite design was performed based on single factor experiments and the quadratic regression analysis of the experimental results was conducted by using Design Expert software.The optimum medium composition(g/L) was glucose 100,xylose 50,(NH4)2SO4 3.0 and KH2PO4 0.3,and the optimum culture conditions were medium volume in a 250 mL flask of 50 mL,shaking table speed of 180 r/min,inoculation amount of 12% and culture temperature of 32 ℃.Under these conditions,the concentration of L-lactic acid reached up to 119.216 g/L after 72 h fermentation and the conversion rate was up to 79.48%.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46