作 者：
朱书礼;张迎秋;陈蔚涛;杨计平;李捷;武智;李新辉
摘 要：
为了研究不同鱼类胰α-淀粉酶基因5'端调控序列转录因子差异与鱼类食性分化之间的关系。通过PCR克隆测序和查询NCBI数据库,获得32种鱼类胰α淀粉酶基因5'端824 bp序列,并对鱼类胰α淀粉酶基因5'端序列进行转录因子和系统发育分析。按不同的营养类型将鱼类分为杂食性、植食性和肉食性,通过百分比相似性分析不同食性鱼类的胰α淀粉酶基因转录因子的组成差异以及转录因子与鱼类食性的关系。结果显示:不同食性鱼类的胰α-淀粉酶基因5'端序列存在转录因子种类差异,植食性-肉食性鱼类差异主要体现在E47、C/EBPalpha、NF-Y和Pax-2,植食性-杂食性差异主要体现在deltaEF1、MyoD、NF-Y、AREB6和Pax-2,杂食性-肉食性差异主要体现在GATA-1、SRY、MyoD、HFH-8、AREB6、Pax-2、STAT5A和AP-1。系统发育结果与传统形态分类学大体相符,相同食性的鱼类并没有聚为一类。鱼类胰α-淀粉酶基因5'端调控序列中与食性分化相关的转录因子有E47、C/EBPalpha、NF-Y、Pax-2、deltaEF1、MyoD、AREB6、GATA-1、SRY、HFH-8、STAT5A和AP-1;胰α-淀粉酶基因5'端序列的转录因子与鱼类食性分化具有一定的关系。
译 名：
Analysis of the relationship between the pancreatic alpha amylase gene 5' flanking sequence and the feeding habits of fish
作 者：
ZHU Shuli;ZHANG Yingqiu;CHEN Weitao;YANG Jiping;LI Jie;WU Zhi;LI Xinhui;Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences;
关键词：
feeding habits;;fish;;amylase gene;;5' flanking sequence
摘 要：
In this study, we aimed to examine the relationship between the pancreatic alpha-amylase gene 5' flanking sequence and the feeding habits of fish by analyzing the differences of alpha-amylase gene transcription factors in different fishes. Pancreatic alpha-amylase gene 5' flanking sequences were obtained from a total of 32 fish species. Five of them were obtained by PCR and the remaining were obtained by searching the NCBI database. The length of the obtained 5' flanking sequences was 824 bp. Then, the transcription factors and phylogenetic relationship analyses were carried out for these pancreatic alpha-amylase gene 5' flanking sequences. Fishes were divided into herbivores, omnivores, and carnivores, according to their different trophic positions. The percentage similarity analysis was used to study the differences in the composition of transcription factors in fishes with different feeding habits. The similarity of the pancreatic alpha-amylase gene 5' flanking sequence among different fishes was very low. The pancreatic alpha-amylase gene 5' flanking sequences of 32 fish species contained a species transcription factor that ranged from 40 to 98. The results of the percentage similarity analysis showed that there were differences in the transcription factors of pancreatic alpha-amylase gene 5' flanking sequences among fishes with different feeding habits. Differences between the Herbivore and Carnivore groups were mainly found in the E47, C/EBPalpha, NF-Y, and Pax-2 transcription factors. Differences between the Herbivore and Omnivore groups were mainly found in the deltaEF1, MyoD, NF-Y, AREB6, and Pax-2 transcription factors. Differences between the Omnivore and Carnivore groups were mainly found in the GATA-1, SRY, MyoD, HFH-8, AREB6, Pax-2, STAT5 A, and AP-1 transcription factors. These transcription factors play an important role in glucose metabolism and can enhance or inhibit gene expression. In liver glucose and lipid metabolism, the E47 and GR transcription factors act jointly as metabolism promoters and enhancers. In the regulation of gluconeogenesis, C/EBPalpha and FOXO1 act together on the IRE controlled promoter to regulate the expression of target genes such as PCK1 and G6 PC. Transcription factor NF-Y plays an important role in promoting glucose metabolism. The phylogenetic tree results were generally consistent with the traditional morphological taxonomy. Fishes with the same feeding habits did not cluster together in the phylogenetic tree. The results indicated that there were several transcription factors in the pancreatic alpha-amylase gene 5' flanking sequences that were related to the feeding habits of fish. There is a certain relationship between transcription factors and the dietary differentiation of fish.
