Position: Home > Articles > The Screening and Identification of Endophytic Bacteria of Amorphophallus konjac against Erwinia carotovora var. carotovora
Journal of Yunnan Agricultural University(Natural Science)
2015,30
(4)
547-553
防治软腐病的魔芋内生细菌的筛选与鉴定
作 者：
周丽洪;李淼;姬广海;王永吉;杨俊;魏兰芳
单 位：
云南农业大学;云南农业大学农科基础实验教学中心
关键词：
魔芋内生细菌;魔芋细菌性软腐病;生物防治;鉴定
摘 要：
本研究采用相似生境分离方法分离魔芋内生细菌后经室内平板初筛、温室复筛试验筛选获得对魔芋软腐病具有良好防治效果的生防菌。研究共分离出127株内生细菌,经过多重筛选后获得3株抑菌效果较好的生防菌(M3,M2,1-7)。经培养性状、菌落形态观察、生理生化特性分析(Biolog系统)和16S r DNA序列分析等方法,将M3,M2,1-7分别鉴定为:枯草芽孢杆菌(Bacillus subtilis)、解淀粉芽孢杆菌(Bacillus amyloliquefaciens)和成团泛生菌(Pantoea agglomerans)。这是将寄主植物内生细菌作为生防菌防治该病害的首次报道。
译 名：
The Screening and Identification of Endophytic Bacteria of Amorphophallus konjac against Erwinia carotovora var. carotovora
作 者：
ZHOU Lihong;Li Miao;JI Guanghai;WANG Yongji;YANG Jun;WEI Lanfang;Key Laboratory of Agricultural Biodiversity for Plant Disease Management under the Ministry of Education,Yunnan Agricu Ltural University;College of Bioengineering,Sichuan University of Scionce and Engineering;Experimental Agricultural Center,Yunnan Agricultural University;
关键词：
endophytic bacteria;;Pectobacterium carotovora subsp.carotovora;;Amorphophallus konjac;;biological control;;identification
摘 要：
In this study,127 endophytic bacteria of konjac were isolated by similar habitat separation method. Three strains( M3,M2,1-7) were screened out for control the soft rot of Amorphophallus konjac by plate screening test and greenhouse rescreening test. The bacteria strains M3,M2,1-7 were identified as Bacilus subtilis,Bacillus amyloliquefaciens and Pantoea agglomerans respectively,according to the characters of cultural colonies,morphological observation,biochemical and physiological identification( Biolog carbon source utilization analysis),16 S r DNA sequences analysis. This is the first report of use host plant endophytic bacteria as biocontrol agents to control the disease.
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