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Position: Home > Articles > Polymorphism Analysis for Exon 2 of Melatonin Receptor 1a Gene in Sheep Journal of Shihezi University(Natural Science) 2008,26 (3) 303-306

绵羊褪黑激素受体1a基因第二外显子的多态性分析

作  者:
李广录;赵宗胜;阿米娜;许汉峰;薛安勇
单  位:
石河子大学动物科技学院
关键词:
绵羊;褪黑激素受体;基因;PCR-RFLP
摘  要:
采用2种限制性内切核酸酶MnlⅠ和RsaⅠ对常年发情的湖羊以及季节性发情的中国美利奴羊、罗米丽羊、罗米丽(Romilly Hills)×中国美利奴(新疆军垦型)褪黑激素受体1a(MTNR1A)基因外显子2的824bp扩增产物进行PCR-RFLP分析。结果表明:MTNR1A基因外显子2的在605位碱基处表现出MnlⅠ酶切多态性,在604位碱基处表现出RsaⅠ酶切多态性。χ2适合性检验结果表明4种绵羊MTNR1A基因第二外显子在RsaⅠ和MnlⅠ酶切位点上都达到Hardy-Weinberg平衡状态(P>0.05);4种绵羊在RsaⅠ和MnlⅠ酶切位点上各基因型的分布通过χ2独立性检验,结果表明差异极显著(P<0.01)。
译  名:
Polymorphism Analysis for Exon 2 of Melatonin Receptor 1a Gene in Sheep
作  者:
LI Guang-lu1,2,ZHAO Zong-sheng1,A-mina1,XU Han-feng1,XUE Aan-yong1(1 College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang 832003,China;2 College of Animal Science and Technology,Henan University of Science and Technology,Luoyang,Henan,471003,China)
关键词:
sheep;melatonin receptor;gene;PCR-RFLP
摘  要:
The exon 2 of melatonin receptor 1a(MTNR1A) gene was amplified and a specific fragment of 824bp was obtained in 4 sheep including non-seasonal estrous breeds(Hu sheep) and seasonal estrous breeds(China Merino、Romilly Hills and Romilly Hills×China Merino Sheep).The 824bp PCR product was digested with restriction endonucleases Mnl Ⅰ and Rsa Ⅰ,and the genetic polymorphism was detected by PCR-RFLP.The results showed that polymorphic Mnl Ⅰsite was detected at base position 605 of the exon 2 of MTNR1A gene,and polymorphic Rsa Ⅰsite was detected at base position 604.At Mnl Ⅰsite and Rsa Ⅰsite of the exon 2 of MTNR1A gene in 4 sheep breeds,the results of χ2 fitness test indicated that they were all in Hardy-Weinberg equilibrium(P>0.05).The results of χ2 independence test indicated that the distribution and constitution of genetypes at Mnl Ⅰsite and Rsa Ⅰsite of the exon 2 of MTNR1A gene showed significant differences among the 4 sheep breeds(P<0.01).

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