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Position: Home > Articles > Study on Aqueous Two-phase System Extraction of Ginger Protease Food and Nutrition in China 2010 (11) 50-54

生姜蛋白酶的双水相法萃取研究

作  者:
谢芳;唐晓珍;杜新永;卢会忠
单  位:
山东省莱芜市莱城区农业局;山东农业大学食品科学与工程学院
关键词:
双水相萃取;生姜蛋白酶;酶活力;分配系数
摘  要:
以生姜为原料,研究双水相萃取技术分离提取生姜中生姜蛋白酶的提取条件。利用单因素试验与正交试验进行优化,得到最佳萃取方案为PEG分子量为4000,PEG浓度为20%,缓冲盐pH为6.5,缓冲盐浓度为10%。此双水相体系的上相酶活回收和纯化倍数都是最高,分别达279.05%和1.86。
译  名:
Study on Aqueous Two-phase System Extraction of Ginger Protease
作  者:
XIE Fang1,TANG Xiao-zhen1,DU Xin-yong1,LU Hui-zhong2(1Food Science and Engineering College of Shandong Agricultural University,Taian 271018)(2LaiCheng Section Agriculture Bureau in LaiWu Sity of Shandong,Laiwu 271100)
关键词:
Aqueous Two-phase System(ATPS);Ginger Protease(GP);GP Activity;Ratio
摘  要:
Ginger as raw material,extraction technology of ginger protease by Aqueous Two-phase System was studied using single factor and orthogonal experiments.Results indicated that the optimum extraction condition was PEG molecular weight 4000,PEG concentration 20%,pH of buffer salt 6.5 and buffer salt concentration 10%.Under above extraction conditions,the GP activity recovery and purification factor were both highest,reached 279.05% and 1.86 respectively.

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