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当前位置: 首页 > 文章 > 复硝酚钠与GA_3复配对美国红栌组培苗生长的影响 南方农业学报 2017 (9) 1653-1658
Position: Home > Articles > Effects of combination sodium nitraphenolate+GA_3 on Cotinus coggygria tissue culture plants Journal of Southern Agriculture 2017 (9) 1653-1658

复硝酚钠与GA_3复配对美国红栌组培苗生长的影响

作  者：
杨艳敏;贾海丽;潘一展
单  位：
商丘学院风景园林学院
关键词：
美国红栌;组培苗;复硝酚钠;赤霉素(GA3);复配
摘  要：
【目的】探讨生长调节剂复硝酚钠与赤霉素(GA_3)复配对美国红栌组培苗生长的影响,为缩短美国红栌组培苗培养周期提供技术支持。【方法】在B5培养基中分别添加不同浓度的复硝酚钠+GA_3复配液培养美国红栌组培苗,25 d后统计各处理组培苗的单株叶片数、株高、鲜重、干重、生根率及根系条数,分析复硝酚钠+GA_3复配对美国红栌组培苗生长的影响。【结果】0.020 mg/L复硝酚钠+30.0 mg/L GA_3复配液处理的美国红栌组培苗生长效果最佳,单株叶片数、株高、鲜重和干重均显著高于对照(CK)及其他浓度处理(P<0.05),其中,分别比CK提高83.3%、71.8%、86.6%和85.8%,生根效果也较优,生根率最高,达95.2%,且根系条数较多,叶片肥厚、健壮,植株长势良好。相关性分析结果表明,复硝酚钠+GA_3复配液浓度与美国红栌组培苗的单株叶片数、株高、鲜重、干重、根系条数和生根率呈极显著正相关(P<0.01)。【结论】在B5培养基中添加0.020 mg/L复硝酚钠+30.0 mg/L GA_3复配液,可促进美国红栌组培苗生长,进而缩短其培养周期,加快种苗繁育速度。
译  名：
Effects of combination sodium nitraphenolate+GA_3 on Cotinus coggygria tissue culture plants
作  者：
YANG Yan-min;JIA Hai-li;PAN Yi-zhan;Landscape Architecture College,Shangqiu University;
单  位：
Landscape Architecture College,Shangqiu University
关键词：
Cotinus coggygria;;tissue culture plant;;sodium nitraphenolate;;gibberellin(GA3);;combination
摘  要：
【Objective】Effects of plant growth regulator combination sodium nitraphenolate+gibberellin(GA_3)at different concentrations on the growth of Cotinus coggygria were explored in order to provide technical support for shortening the culture cycle of tissue culture plants.【Method】B5 medie adding sodium nitraphenolate+GA_3 of different combinations were used to culture C.coggygria.Leaf number per plant,plant height,fresh weight,dry weight,rooting rate and root number were investigated after 25 d of culture to study the effects of adding sodium nitraphenolate+GA_3 on the tissue culture plants.【Result】0.020 mg/L sodium nitraphenolate+30.0 mg/L GA_3 had the optimal effects on C.coggygria tissue culture plants.Leaf number per plant,plant height,fresh weight,dry weight were significantly higher than those in control(CK)and other treatments(P<0.05).Leaf number per plant,plant height,fresh weight,dry weight increased 83.3%、71.8%、86.6% and 85.8% respectively compared with CK.Rooting was also better than CK and the highest rooting rate reached 95.2%.The plants were strong with large root number,thick leaves and sound growth state.Correlation analysis indicated that there was extremely positive correlation between nitraphenolate + GA_3 concentrations and leaf number per plant,plant height,fresh weight,dry weight,rooting rate and root number(P<0.01).【Conclusion】Adding 0.020 mg/L sodium nitraphenolate + 30.0 mg/L GA_3 into B5 medium can improve the growth for C.coggygria tissue culture plants,and shorten the culture cycle and speed up the breeding process.

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