中国农业期刊集成服务平台

当前位置: 首页 > 文章 > 灵芝保健品中三萜化合物HPLC分离条件的优化安徽农业科学 2010,38 (24) 13029-13031

Position: Home > Articles > Optimization of Separation Condition of Triterpenoids from Ganoderma lucidum under HPLC Journal of Anhui Agricultural Sciences 2010,38 (24) 13029-13031

灵芝保健品中三萜化合物HPLC分离条件的优化

作者：

Ding Fang-li

单位：

Hunan Biological and Electromechanical Polytechnic,Changsha,Hunan 410127,Chin

关键词：

triterpenoids;ganoderma lucidum;extraction solvents;kinds;the health;hpl

摘要：

[目的]优化灵芝中三萜化合物HPLC法的分离条件。[方法]用RP-HPLC法,考察不同提取剂、不同梯度洗脱、不同进样量对灵芝中三萜类化合物分离的影响,同时在最佳条件下分离测定4种市售保健品中三萜类组分。[结果]灵芝中三萜化合物提取剂为氯仿;优选出的灵芝三萜组分的HPLC分离条件为:流动相A为0.2%乙酸-水,流动相B为乙腈,梯度洗脱;检测波长为254nm;进样量为10μl;市售的4种灵芝保健品中只有2种保健品的HPLC分离图谱能与灵芝原药材对应。[结论]该试验得到分离灵芝中的三萜类组分的最佳分离条件,同时对4种市售保健品中三萜类组分进行了测定,此法可用于灵芝提取物的质量控制和其保健品的鉴定。

译名：

Optimization of Separation Condition of Triterpenoids from Ganoderma lucidum under HPLC

作者：

DING Fang-lin (Hunan Biological and Electromechanical Polytechnic,Changsha,Hunan 410127)

关键词：

Ganoderma lucidum ; Triterpenoids; HPLC

摘要：

[Objective]The aim was to optimize the separation condition of triterpenoids from Ganoderma lucidum. [Method]The effects of different extraction solvents,different mobile phases and different injecting amount on the separation of triterpenoids were studied by RP-HPLC,and the triterpenoids of 4 kinds of health care products were determined under the optimum condition. [Result]The optimum condition were as follows:extraction solvents of chloroform,aqueous phase of 0.2% acetic acid-water,injecting amount of 10-30 ul,and the chromatogram of 2 kinds of the health care products were similar with that of Ganoderma lucidum medicinal material. [Conclusion]The optimum conditions were obtained,and the 4 kinds of health care products were determined by this method,which could used to control the extraction quality of Ganoderma lucidum and evaluate the health care products.

相似文章

Research status and progress of the triterpenoids in Ganoderma lucidum. [Ye Li, Zhu ZhongMin, Yao WeiXi, Ruo-Yun Che] 药用植物研究(英文版) 2012,3 (12)
Study on the extraction technology of triterpenoids from Ganoderma applanatum (Pers.) Pat. [Lin QianQian, Shujie Zhao, Zhongmei H] 药用植物研究(英文版) 2012,3 (11)
Research progress on chemical compositions and pharmacological activities of Pyrrosia plants. [Lijun Chen, Yongjie Ma, Yupeng Li, Yan Zhang, Fuhong He, Ye‐Gao Che] 药用植物研究(英文版) 2011,2 (3)
HPLC-DAD-ELSD simultaneous determination of four triterpenoids in RHIZOMA ALISMATIS. [Qiu JianFang, Lin Jing, Xu Wen, Mingqing Huang, Wenming Zhao, Luo FenRong, Xiaoyan Li, WU Shuishen] 药用植物研究(英文版) 2014,5 (5)

导出引用

计量

文章访问数: 15

HTML全文浏览量: 0

PDF下载量: 0

灵芝保健品中三萜化合物HPLC分离条件的优化

相似文章

所属期刊

安徽农业科学

推荐期刊

药用植物研究(英文版)