译 名：
Insecticidal activity and virulence of the transgenic Enterobacter cloacae with the insecticidal tcdA1B1 genes against four agricultural pests
作 者：
ZHANG Peng-Bo1,XU Cai-Xia2,QIU Xue-Hong,HAN Ri-Chou2(1.College of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China;2.Guangdong Entomological Institute,Guangzhou 510260,China)
关键词：
Entomopathogenic bacteria;transgenic Enterobacter cloacae;Spodoptera exigua;Spodoptera litura;Phyllotreta striolata;Locusta migratoria manilensis
摘 要：
The gene of tcdA1B1 is important insecticidal gene from entomopathogenic bacteria Photorhabdus luminescens.The pathogenicity of the transgenic Enterobacter cloacae with tcdA1B1 genes toward four agricultural insect pests (Spodoptera exigua,Spodoptera litura,Phyllotreta striolata,and Locusta migratoria manilensis) was tested in this study.The transgenic E.cloacae showed variable virulence to the four insect pests.The mortalities of the 2nd instar larvae of S.exigua and S.litura,the adults of P.striolata,and the larvae of L.migratoria manilensis were 80.05%,81.83%,72.31%,and 39.74% after 11 d,11 d,15 d,19 d,respectively,and their LT50 values were 8.64 d,8.38 d,11.04 d,and 28.22 d,respectively,at the bacterial concentration of 5.0 ×108 cfu/mL,by leaf dip method.This transgenic E.cloacae showed high insecticidal activity to S.exigua,S.litura and P.striolata and may be used for the potential biological control of these insect pests in the field.
