Position: Home > Articles > Role of phoP Regulon in Environmental Stress Tolerance in Cronobacter sakazakii
FOOD SCIENCE
2019
(10)
50-56
phoP调节子对阪崎克罗诺肠杆菌环境耐受力的影响
作 者：
周亚琴;李梦云;夏效东
单 位：
西北农林科技大学食品科学与工程学院
关键词：
phoP基因;阪崎克罗诺肠杆菌;环境耐受
摘 要：
为研究克阪崎罗诺肠杆菌中phoP基因与环境耐受力的关系,采用Red同源重组方法构建阪崎克罗诺肠杆菌的phoP基因缺失菌株,并从生长曲线、酸碱耐受、温度耐受等方面,研究phoP基因缺失前后阪崎克罗诺肠杆菌环境耐受力的变化。结果表明:phoP基因缺失菌株的生长速度明显延缓,该基因的缺失减弱了菌对外界酸碱和高温的耐受性,并缩短了热致死时间;phoP基因缺失菌株抵御胆盐环境的能力亦有所下降,与野生型菌株相比,它在4%、8%、12%胆盐溶液环境中的存活率分别降低了19.93%(P<0.05)、40.73%(P<0.01)和45.84%(P<0.01)。此外phoP基因缺失菌株对渗透压的耐受力与野生株相比也显著降低(P<0.05)。综合以上结果表明,phoP基因有助于阪崎克罗诺肠杆菌应对外界环境刺激。
译 名：
Role of phoP Regulon in Environmental Stress Tolerance in Cronobacter sakazakii
作 者：
ZHOU Yaqin;LI Mengyun;XIA Xiaodong;College of Food Science and Engineering, Northwest A&F University;
单 位：
ZHOU Yaqin%LI Mengyun%XIA Xiaodong%College of Food Science and Engineering, Northwest A&F University
关键词：
phoP;;Cronobacter sakazakii;;environmental?tolerance
摘 要：
The?aim?of?this?study?was?to?investigate?the?effect?of?the? phoP?regulon?on?environmental?stress?tolerance?in?Cronobacter sakazakii. A phoP-deleted?strain?of C. sakazakii was?constructed?by?using?the?Red?homologous?recombination?method,?and?then?the?growth?curve?and?stress?tolerance?of?the?phoP-deleted?strain?were?examined.?The?results?showed?that?the?phoP-defective?strain?showed?delayed?growth?and?weaker?tolerance?to?external?acid-base?and?heat?stress.?Meanwhile,?it?exhibited?shortened?time?of?death?from?heat?treatment.?phoP?rendered C. sakazakii?tolerant?to?bile?salt?environment,?and?its?deletion?resulted?in?a?reduction?in?survival?rate?of?19.93%(P < 0.05), 40.73%(P?
导出引用
计量
文章访问数: 5
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
