作 者：
何永明;丁秀英;丛林晔;郭光;陈淑君;张军
单 位：
中国农业大学植物生理学与生物化学国家重点实验室;中绿华夏有机食品认证中心;北京移栽灵科技有限责任公司
关键词：
β-氨基丁酸(BABA);烟草花叶病毒(TMV);诱导抗性;病原相关蛋白(PR)
摘 要：
本文研究了β-氨基丁酸(BABA)诱导系统侵染寄主烟草NC89产生PR蛋白及其对TMV的抑制作用。用5mmol/LBABA对抗性烟和非抗性烟进行叶面喷施处理均可以抑制烟草叶片中TMV的产生,平均抑制率分别达到59%和49%。BABA和SA处理均可以诱导2种烟草叶片中PR1、PR2和PR5基因的表达,其中对PR1的诱导作用相对较强。分别用BABA和SA处理非抗性烟草,在处理后的不同时间内2种药剂对PR1基因的诱导规律基本相同。用BABA和SA分别预处理非抗性烟草2d后接种TMV,2种药剂对烟草叶片中PR1基因的影响是完全不同的,其中,BABA处理后接种TMV抑制PR1基因的表达,在接种后第72h已经检测不到PR1基因的存在,SA处理后接种TMV对PR1基因的影响呈现弱-强-弱的变化趋势,在接种后第24hPR1的表达量达到最强,以后逐渐减弱。试验结果表明:BABA能够提高非抗性烟草对TMV的抗性,但是BABA诱导烟草产生抗TMV的作用机制可能是一种不同于目前普遍公认的SA的作用机制。
译 名：
Induction of PR protein and inhibition of TMV in tobacco by β-amino-butyric acid
作 者：
HE Yong-ming1, DING Xiu-ying2, CONG Lin-ye3, GUO Guang1, CHEN Shu-jun2, ZHANG Jun1 (1State Key Laboratory of Plant Physiology and Biochemistry of China Agricultural University, Beijing 100094, China; 2Beijing Yizailing Science and Technology Co., LTD, Beijing 100094, China; 3China Organic Food Certification Center, Beijing 100081, China)
关键词：
β-amino-butyric acid (BABA); Tobacco mosaic virus (TMV); induced-resistance; pathogenesis-related protein (PR)
摘 要：
This is the first research on induction of PR protein and inhibition of TMV in Nicotiana tabacum cv. NC89 by β-amino-butyric acid (BABA). 5 mmol/L BABA induced significant anti-TMV activity in Nicotiana tabacum cv. Samsun NN and N. tabacum cv. NC89. The average inhibiting effects of BABA on TMV was 59% and 49% respectively. The expressions of PR1 , PR2 and PR5 genes were investigated by Northern blotting at different hours after treatment with BABA and salicylic acid (SA, as a control) respectively. BABA or SA treatment could both induce and enhance the expression of PR1,PR2 and PR5 gene in tobacco leaves, and induction effect of PR1 was better than that of the other two genes. The same changes of PR1 gene induced were observed in N. tabacum cv. NC89 after BABA and SA treatment. There were complete differences of PR1 gene expression in N. tabacum cv. NC89 infected by TMV after 2 d pre-treated by BABA and SA. TMV inoculation after BABA pre-treatment inhibited the expression of PR1 gene, however, SA pre-treatment induced the expression. The results suggested that BABA could enhance anti-TMV activity in N. tabacum cv. NC89. However, the mechanism of BABA-induced resistance against TMV was different from that of SA in N. tabacum cv. NC89.
