Position: Home > Articles > Detection of Quinolones in Hotpot Seasoning by Solid Phase Extraction-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
Journal of Anhui Agricultural Sciences
2020
(6)
184-187+209
通过型固相萃取-超高效液相色谱-串联质谱法检测火锅底料中的喹诺酮类药物
作 者：
沈雄雅;何晓明;吴丹虹;叶冰洁;闻佳钰;刘强欣
单 位：
绿城农科检测技术有限公司
关键词：
通过型固相萃取-超高效液相色谱-串联质谱;火锅底料;喹诺酮类药物
摘 要：
[目的]建立通过型固相萃取-超高效液相色谱-串联质谱法测定火锅底料中11种喹诺酮类药物的分析方法。[方法]样品用酸化乙腈提取,经Oasis Prime HLB固相萃取柱净化,收集流出液,用超高效液相色谱-串联质谱仪多反应监测扫描模式检测和同位素内标定量。[结果]所有目标物在线性范围内线性良好,相关系数≥0.99,检出限为0.6～4.8μg/kg,定量限为2.0～13.2μg/kg,不同加标水平的平均回收率为78.5%～103.4%,相对标准偏差为2.8%～9.1%。[结论]该方法具有快速、准确、灵敏度高的特点,可用于火锅底料中11种喹诺酮类药物的测定。
译 名：
Detection of Quinolones in Hotpot Seasoning by Solid Phase Extraction-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
作 者：
SHEN Xiong-ya;HE Xiao-ming;WU Dan-hong;Green City Agricultural Detection Technology Co.,Ltd.;
关键词：
Solid phase extraction-ultra performance liquid chromatography-tandem mass spectrometry;;Hotpot seasoning;;Quinolones
摘 要：
[Objective]The research aimed to establish an analytical method for the determination of 11 quinolones in hotpot seasoning by solid phase extraction-ultra performance liquid chromatography-tandem mass spectrometry.[Method]Samples were extracted with acidic acetonitrile,cleaned-up by solud-phase extraction using Oasus Prime HLB column. The analytes were detected in the multi-reaction monitoring(MRM) mode and quantified by the isotope internal standard method. [Result]The calibration curves were linear well in the corresponding concentration ranges,with correlation coefficient ≥ 0.99. The limits of detection and quantification were 0.6-4.8 μg/kg and 2.0-13.2 μg/kg,respectively.The average recoveries at three spiked levels ranged from 78.5% to 103.4% with RSD of 2.8%-9.1%.[Conclusion]This method is rapid,accurate and sensitive.It can be used for the determination of 11 quinolones in hotpot seasoning.
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