作 者：
贺贺千;库尔班·吐拉克;华兴耀;华兴光;李彦林;曹英;金大智;杨玮
单 位：
新疆昌吉市盛华马鹿繁育基地;新疆农业大学动物科学学院
关键词：
马鹿;同期排卵-定时输精;同期发情;不返情率;激素含量
摘 要：
本研究旨在探讨不同同期排卵-定时输精程序对马鹿繁殖效果的影响。选用40只健康、年龄相近的经产天山马鹿母鹿,随机分为5组,每组8只,对照组在马鹿发情周期内放入孕酮阴道栓(CIDR),在埋栓第12天取出CIDR,同时肌注孕马血清促性腺激素(PMSG),撤栓48 h后肌注促排3号(A_3),再过12 h后人工输精,同时肌注促排3号(A_3)。试验组在马鹿发情周期内肌注促性腺激素释放激素(GnRH)和维生素AD注射液,在肌注第7天再肌注前列腺素(PG),试验1组在48 h后第2次肌注GnRH和维生素AD注射液,16～24 h后人工输精;试验2组在48 h后第2次肌注GnRH和维生素AD注射液,同时人工输精;试验3组在72 h后第2次肌注GnRH和维生素AD注射液,同时人工输精;试验4组在56 h后第2次肌注GnRH和维生素AD注射液,16 h后人工输精。在处理前和人工授精时采集血样,测定血清中孕酮(P_4)、雌激素(E_2)、促卵泡素(FSH)、促黄体素(LH)的含量。结果表明,对照组、试验1组、试验2组和试验4组同期发情率(分别为100%、87.5%、87.5%、100%)极显著高于试验3组(37.5%)(P<0.01),但对照组和试验3组母鹿不返情率(分别为75.0%、75.0%)极显著高于试验1组、试验2组、试验4组(分别为12.5%、37.5%、25.0%)(P<0.01);各组处理后(输精时)的母鹿血清中E_2、LH、P_4和FSH含量整体极显著高于处理前(P<0.01),但各组之间无显著差异(P>0.05);试验组方法处理母鹿成本显著低于对照组。采用试验组方法处理母马鹿可保证较高的不返情率,且操作简单、成本低、有效避免母鹿反复麻醉损伤等优点,优于对照组方法。
译 名：
Effects of Different Procedures of Ovulation Timing Insemination on Reproduction of Wapiti
作 者：
HE He-qian;Kuerban Tulake;HUA Xing-yao;HUA Xing-guang;LI Yan-lin;CAO Ying;JIN Da-zhi;YANG Wei;College of Animal Sciences,Xinjiang Agricultural University;Changji Shenghua Domesticated Wapiti Breeding Base;
关键词：
red deer(Cervus elaphus);;simultaneous ovulation-scheduled insemination;;estrus synchronization;;non-return rate;;hormone content
摘 要：
The aim of this study is to explore the effects of different ovulation timing insemination procedures on the reproduction of red deer(Cervus elaphus).40 healthy and same-aged female deer from Tianshan Mountain were selected and randomly divided into 5 groups with 8 in each group. In the control group,CIDR was put into the vagina of the horses during the estrus cycle,during which,pregnant mare serum gonadotropin(PMSG) was injected intramuscularly into the pregnant horses and removed on the 12 th day later and 48 h after removing the CIDR,A_3 was injected. After another 12 h,artificial insemination was conducted and at the same time,A_3 was injected intramuscularly. In the experimental group,gonadotropin-releasing hormone(GnRH) and vitamin AD were injected intramuscularly during estrus cycle, and on the 7 th day after the injection, prostaglandin(PG) was injected again; In experimental group 1,gonadotropin-releasing hormone(GnRH) and vitamin AD were injected intramuscularly the second time 48 h later,and 16-24 hlater,artificial insemination was conducted;In experimental group 2,gonadotropin-releasing hormone(GnRH)and vitamin AD were injected intramuscularly the second time 48 hlater,and at the same time,artificial insemination was conducted;In experimental group 3,gonadotropin-releasing hormone(GnRH)and vitamin AD were injected intramuscularly the second time 72 hlater,and at the same time,artificial insemination was conducted;In experimental group 4,gonadotropin-releasing hormone(GnRH)and vitamin AD were injected intramuscularly the second time 56 hlater,and artificial insemination was conducted 16 hlater.Blood samples were collected before and during artificial insemination,and serum content of progesterone(P_4),estrogen(E_2),follicle stimulating hormone(FSH)and luteinizing hormone(LH)were measured.The results showed that the estrus synchronization rates of the control group,test group 1,test group 2 and test group 4(100%,87.5%,87.5% and 100%)were significantly higher than that of test group 3(37.5%)(P <0.01),but the non-return rates of control group and test group 3(75.0%,75.0%)were significantly higher than those of test group 1,test group 2 and test group 4(12.5%,37.5%,25.0%)(P <0.01);The overall levels of E2,LH,P4 and FSH in the maternal deer serum after treatment(at infusion)in each group were significantly higher than those before treatment(P <0.01),but there was no significant difference between the groups(P >0.05);the cost of treatment of maternal deer in the experimental group was significantly lower than that in the control group.The above results suggest that using the test group method to treat the female deer(Cervus elaphus)can ensure a high non-return rate,and the advantages of simple operation,low cost and effective avoidance of repeated anaesthesia damage than those of the control group.
