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当前位置: 首页 > 文章 > IGF-1在不同鸡与鹌鹑杂交禽胚胎肌肉发育过程中的表达规律与差异 新疆农业科学 2012,49 (9) 1717-1722
Position: Home > Articles > Developmental Expressional Profiles of IGF-1 during Embryonic Myogenesis in Two Strains of Hybrid of Chicken - Quail Xinjiang Agricultural Sciences 2012,49 (9) 1717-1722

IGF-1在不同鸡与鹌鹑杂交禽胚胎肌肉发育过程中的表达规律与差异

作  者：
梁耀伟;赵宗胜;陈丹盈;赵松华;张慧;班谦
单  位：
石河子大学动物科技学院
关键词：
鸡;鹌鹑;杂交禽;肌肉发育;IGF-1基因;实时荧光定量PCR
摘  要：
【目的】研究IGF-1基因在蛋用型种鸡(♂)×鹌鹑(♀)和肉用型种鸡(♂)×鹌鹑(♀)两种不同杂交禽胚胎肌肉发育过程中的表达规律,比较该基因在两种不同杂交禽中的差异表达,探讨IGF-1基因在肌肉发育网络调控中的作用。【方法】通过人工受精的方法,蛋用型种鸡(♂)×鹌鹑(♀)获得蛋杂交禽蛋、肉用型种鸡(♂)×鹌鹑(♀)获得肉杂交禽蛋,与鸡蛋、鹌鹑蛋按照鸡标准孵化条件同批人孵,采集第7～17 d活胚胸肌组织,应用实时荧光定量PCR技术检测IGF-1基因在四个物种中每个时间点mRNA的相对表达量。【结果】IGF-1基因在胚胎肌肉发育的第7～17 d均有表达,在第10 d达到第一次峰值,鸡和鹌鹑在第16 d达到第二次峰值,蛋杂交禽和肉杂交禽在第15 d达到峰值。【结论】IGF-1可能在胚胎肌肉的生长发育过程中起调控作用;IGF-1 mRNA表达规律与成肌细胞增殖和肌管融合规律一致,可能参与调控成肌细胞的增殖和肌管的融合。
译  名：
Developmental Expressional Profiles of IGF-1 during Embryonic Myogenesis in Two Strains of Hybrid of Chicken - Quail
作  者：
LIANG Yao - wei,ZHAO Zong - sheng,CHEN Dan - ying,ZHAO Song - hua,ZHANG Hui,BAN Qian (College of Animal Science and Technology,Shihezi University,Shihezi Xinjiang 832003,China)
关键词：
chicken;;quail;;hybrid;;myogenesis;;IGF - 1;;real - time quantitative PCR
摘  要：
[Objective]The purpose of this study was to analyze IGF - 1 mRNA expression profiles and differences during embryonic myogenesis in two strains of hybrid of chicken - quail,and evaluate the potential relationships between IGF - 1 expression and myogenesis.[Method]Two strains of cross -bred eggs of quail ($ ) were acquired by artificial insemination by chicken(6 ) - semen,with the eggs of chicken,quail and hybrid in one batch hatched according to the standard condition of chicken,and then the breast muscle tissues from day 7 to day 17 were collected.The expression of IGF - 1 mRNA in different species every day was revealed by real - time quantitative PCR.[Result]The expression of IGF - 1 mRNA was detected from day 7 to day 17.The first peak time of IGF - 1 expression was noticed at day 10 in four species,the second peak time was noticed at day 16 in Chicken and Quail,and at day 15 in Meat - Hybrid and Egg - Hybrid. [Conclusion]IGF - 1 maybe plays a key role in the process of myogenesis.The expression profile of IGF - 1 was in accord with the profile of myoblasts proliferation and muscle tube fusion,and it maybe plays a key role in the process of myoblasts proliferation and muscle tube fusion.

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