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Position: Home > Articles > Transgenic Pig Construction of the Inducible IGF-1 Model Hubei Agricultural Sciences 2015,54 (20) 5077-5079+5083

诱导型IGF-1转基因猪的构建

作  者:
刘西梅;华再东;李莉;张立苹;肖红卫;任红艳;毕延震
单  位:
湖北省农业科学院畜牧兽医研究所/动物胚胎及分子育种湖北省重点实验室
关键词:
诱导型;IGF-1;转基因猪
摘  要:
以显微注射的方法,将去掉原核DNA的Pc DNA3.1IGFTREtr TA诱导型IGF-1载体注射到猪受精卵原核中,经胚胎移植、怀孕、产仔、取样、检测等过程,得到幼仔85头,其中的11头为PCR检测阳性,进一步的Southern blot检测有10头阳性,即构建的原代诱导型IGF-1转基因猪有10头。
译  名:
Transgenic Pig Construction of the Inducible IGF-1 Model
作  者:
LIU Xi-mei;HUA Zai-dong;LI Li;ZHANG Li-ping;XIAO Hong-wei;REN Hong-yan;BI Yan-zhen;Hubei Academy of Agriculture Science/Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding;
关键词:
inducible;;IGF-1;;transgenic pig
摘  要:
In this study, the pc DNA3.1IGFTREtr TA induced IGF-1 vector without prokaryotic DNA was microinjected into pronucleus of pig fertilized eggs. Through embryo transfer, pregnancy, parturition, sampling and testing procedures, 85 little life born, of which 11 was tested positive for PCR, 10 positive for Southern blot detection. It indicated that there had 10 primary transgenic pigs of inducible IGF-1 in the test.

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