当前位置: 首页 > 文章 > 斜纹夜蛾核型多角体病毒Ⅱ型分离株同源重复区hr5的结构功能分析 山东农业科学 2017 (8) 99-103
Position: Home > Articles > Structure-Function Analysis of Homologous Region 5 from Spodoptera litura Nucleopolyhedrovirus Ⅱ Shandong Agricultural Sciences 2017 (8) 99-103

斜纹夜蛾核型多角体病毒Ⅱ型分离株同源重复区hr5的结构功能分析

作  者:
刘惠芬;衣葵花;刘文光;李智峰;李云芝
单  位:
山东省蚕业研究所
关键词:
斜纹夜蛾;核型多角体病毒;同源重复区;增强子
摘  要:
对斜纹夜蛾核型多角体病毒Ⅱ(Splt NPVⅡ)基因组DNA中的同源重复区hr5的结构功能进行研究。结果表明,Splt NPVⅡhr5大小为389 bp,含有3个64 bp不完全回文序列,回文序列的中心均含有1个PvuⅠ限制性酶切位点,并且存在1个与病毒基因组DNA复制相关的motif(CGATT)基序。瞬时表达分析表明,hr5在野生型病毒感染和未感染的细胞中均具有增强早期基因ie1启动子活性的功能;实时荧光定量PCR结果显示,hr5在野生型病毒作为辅助病毒时,具有基因组DNA复制起始原点的功能。研究证明Splt NPVⅡhr5具有复制起始原点和增强子的双功能作用。
译  名:
Structure-Function Analysis of Homologous Region 5 from Spodoptera litura Nucleopolyhedrovirus Ⅱ
作  者:
Liu Huifen;Yi Kuihua;Liu Wenguang;Li Zhifeng;Li Yunzhi;Shandong Institute of Sericultural Research;
关键词:
Spodoptera litura;;Nucleopolyhedrovirus;;Homologous region(hr);;Enhancer
摘  要:
The structure-function of homlogous region 5( hr5) from Spodoptera litura Nucleopolyhedrovirus Ⅱ( Splt NPVⅡ) was studied in this paper. The results showed that the length of hr5 is 389 bp consisting of three 64-bp imperfect palindromes,and each palindrome contains a PvuⅠ site in the center and one motif( CGATT) which related to the replication of genomic DNA of virus. A transent expression assay demonstrated that the expression of Splt NPVⅡ-ie1 promoter-driven luciferase gene was enhanced by hr5 in both infected and uninfected Spli221 cells. Real-time PCR confirmed that the hr5 could function as ori of viral DNA replication in infected cells with wild type virus as the helper virus. The study results indicated that Splt NPVⅡhr5is bifunctional,having both ori and enhancer activities.

相似文章

计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊