Position: Home > Articles > Extraction and Determination of Polysaccharides in Pyracantha fortuneana
Hubei Agricultural Sciences
2014
(10)
186-189
火棘中水溶性多糖的提取及测定
作 者：
楚红英;陈志冉;李瑜
单 位：
黄河水利职业技术学院环境与化学工程系;郑州人民医院颐和医院泌尿外科
关键词：
火棘(Pyracantha fortuneana);多糖;提取;苯酚-硫酸法
摘 要：
采用超声波辅助提取技术,通过单因素试验和L9(34)正交试验,探讨了提取条件对火棘(Pyracantha fortuneana)中多糖类物质提取率的影响,采用苯酚-硫酸法测定火棘中多糖含量,以葡萄糖作为标准对照物,在482 nm处测定吸光度,并计算其含量。结果表明,在火棘多糖超声波最佳提取工艺条件下(提取剂pH为7,30℃下按液料比30∶1提取40 min),火棘果中多糖提取率达2.148%。
译 名：
Extraction and Determination of Polysaccharides in Pyracantha fortuneana
作 者：
CHU Hong-ying;CHEN Zhi-ran;LI Yu;Department of Environment and Chemical Engineering,Yellow River Conservancy Technical Institute;Department of Urology,Zhengzhou People's Hospital Yi He Hospital;
关键词：
Pyracantha fortuneana;;polysaccharides;;extraction;;phenol-H2SO4colorimetry
摘 要：
The conditions of ultrasound-assisted extracting polysaccharides from Pyracantha fortuneana were studied with single factor analysis and L9(34) orthogonal experiment.The content of polysaccharides in Pyracantha fortuneana was determined by phenol-H2SO4colorimetry at 482 nm with glucose as the standard.The results showed that the optimal conditions of ultrasonic extracting polysaccharides from Pyracantha fortuneana were 30 ℃ for 40 min with pH 7 and the best ratio of liquid(mL) to solid(g) was 30∶1.Under the optimal conditions,the yield of polysaccharides from Pyracantha fortuneana was 2.148%.
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