Position: Home > Articles > Study on the Enzyme Matching Ratio Optimization by the Mixture Design in the Extraction of Lonicera edulis Pigment
Journal of Anhui Agricultural Sciences
2011,39
(19)
589-591
蓝靛果色素提取中混料设计优化酶配比的研究
作 者：
刘德江;刘娟;申健
单 位：
佳木斯大学生命科学学院;佳木斯大学药学院
关键词：
蓝靛果色素;混料设计;优化
摘 要：
[目的]寻求蓝靛果色素提取中果胶酶、纤维素酶、木瓜蛋白酶的最佳配比。[方法]采用混料设计研究了3种酶不同比例对蓝靛果色素提取液吸光度值的影响,建立酶配比与吸光度值之间的回归模型,考查了3种酶组分的互作效应,通过模型优化得到了最优配比。[结果]3种酶的最优配比为果胶酶46.6%、纤维素酶17.6%、木瓜蛋白酶36.0%。[结论]该研究为蓝靛果资源的综合利用提供了理论依据。
译 名：
Study on the Enzyme Matching Ratio Optimization by the Mixture Design in the Extraction of Lonicera edulis Pigment
作 者：
LIU De-jiang et al(College of Pharmacy,Jiamusi University,Jiamusi,Heilongjiang 154007)
关键词：
Lonicera edulis;Mixture design;Optimization
摘 要：
[Objective]The research aimed to find the optimum proportions of pectinase,cellulose enzyme and papain in the extraction of Lonicera edulis pigment.[Method]The influences of different proportions of three enzymes on the absorbance value of Lonicera edulis pigment extraction fluid were studied by the mixture design.The regression model between the enzyme matching ratio and the absorbance value was established,and the interaction effects of three enzymes were studied.By the model optimization,the optimum matching ratio was obtained.[Result]The optimum matching ratios of three enzymes were the pectinase 46.6%,cellulose enzyme 17.6% and papain 36.0%.[Conclusion]The research provided the theory basis for the comprehensive utilization of Lonicera edulis resource.
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