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Position: Home > Articles > Rescue of HEP-Flury Strain of Rabies virus from Cloned cDNA Progress in Veterinary Medicine 2006,27 (2) 103-104

从克隆的cDNA拯救狂犬病毒HEP-Flury株

作  者:
艾军;林宝珍;郭霄峰
单  位:
华南农业大学兽医学院
关键词:
狂犬病毒;反向遗传操作技术;HEP-Flury株
摘  要:
为研究狂犬病毒基因的结构,将狂犬病毒HEP-Flury株全基因组cDNA与N、P、L、G 4个基因的辅助质粒共转染BHK-21细胞,于体积分数为5%CO2培养箱中培养6d,以狂犬病毒N蛋白荧光抗体染色。结果显示,细胞中含有大量的狂犬病毒粒子,表明已建立了狂犬病毒的反向遗传操作系统。
译  名:
Rescue of HEP-Flury Strain of Rabies virus from Cloned cDNA
作  者:
AI Jun,LIN Bao-zhen,GUO Xiao-feng(College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong,510642,China)
关键词:
Rabies virus;reverse genetics;HEP-Flury strain
摘  要:
To study the genes of Rabies virus,the full-length genome cDNA of the HEP-Flury strain and four helper plasmids with N,P,L and G gene respectively were co-transfected to BHK-21 cell.After incubation in 5% CO_(2),the cells were stained with fluorescence antibody of anti-N protein MAb.The results showed that the rabies virus was efficiently recovered from the clone cDNA and Rabies Virus reverse genetic system was developed.

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