中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 柔嫩艾美球虫免疫与鸡盲肠扁桃体TNF-α基因表达动态的关系 中国兽医科学 2008,38 (1) 20-24
Position: Home > Articles > Relationship between Eimeria tenella immunity and kinetic expression of TNF-α gene in chicken cecal tonsil Chinese Veterinary Science 2008,38 (1) 20-24

柔嫩艾美球虫免疫与鸡盲肠扁桃体TNF-α基因表达动态的关系

作  者：
王彩霞;徐赓;王黎霞;安健
单  位：
北京农学院动物科学技术系
关键词：
β-actin基因;柔嫩艾美球虫;盲肠扁桃体;RT-PCR;TNF-α基因
摘  要：
根据GenBank上鸡β-actin、TNF-α的基因序列设计引物,应用逆转录-聚合酶链式反应(RT-PCR)技术克隆获得了β-actin和TNF-α基因,采用β-actin为内参的半定量方法检测TNF-αmRNA在鸡柔嫩艾美球虫免疫前后不同时间的表达情况,以探讨TNF-α基因的表达动态与柔嫩艾美球虫免疫的关系。结果显示,TNF-α基因在两次免疫期间的表达量整体上呈现双峰模式,首免后第9 d达到一个高峰,二免后第7d达到另一个高峰。结果表明,TNF-α在抗球虫感染中有一定的作用。
译  名：
Relationship between Eimeria tenella immunity and kinetic expression of TNF-α gene in chicken cecal tonsil
作  者：
WANG Cai-xia,XU Geng,WANG Li-xia,AN Jian(Department of Animal Science and Technology,Beijing College of Agriculture,Beijing 102206,China)
关键词：
β-actin gene;Eimeria tenella;cecal tonsil;RT-PCR;tumor necrosis factor-α gene
摘  要：
According to chicken β-actin and TNF-α gene sequences in GenBank,primers were designed,and the gene sequences were amplified by reverse transcriptase-polymerase chain reaction(RT-PCR) from the cecal tonsil of chicken experimentally immunized with Eimeria tenella.The expression le-vel of TNF-α mRNA at different time after immunization was detected by semi-quantitative RT-PCR with β-actin gene as internal reference to study the relationship between E.tenella immunization and the kinetic expression of TNF-α gene in chicken cecal tonsil.The results showed that the level of expression of TNF-α gene reached 1st peak on day 9 post-1st-immunization,and reached 2nd peak on day 7 post-booster-immunization.It was concluded that TNF-α had some effect against E.tenella infection.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46