中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > BTH诱导花椰菜对菌核病的抗性研究 植物病理学报 2012,42 (3) 281-289
Position: Home > Articles > Studies on BTH-induced resistance to Sclerotinia sclerotiorum in cauliflower and physiology basis Acta Phytopathologica Sinica 2012,42 (3) 281-289

BTH诱导花椰菜对菌核病的抗性研究

作  者：
孙溶溶;彭真;程琳;朱翔飞;邵泰良;卢钢
单  位：
浙江大学农业与生物技术学院;浙江神良种业有限公司
关键词：
花椰菜;菌核病;BTH;抗性诱导;基因表达
摘  要：
利用苯并噻二唑BTH处理菌核病抗性不同的花椰菜品种幼苗,采用营养生长期活体叶片菌丝块接种鉴定法评价菌核病抗性诱导效果,结果表明经BTH处理的植株菌核病病情指数明显下降,对感病品种和抗病品种的诱抗效果分别达到81.5%和63.8%。对于花椰菜重要的防御酶活性变化研究结果表明,BTH诱导处理的花椰菜植株过氧化物酶(POD)、抗坏血酸酶(SOD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)和多酚氧化酶(PPO)的活性均有所提高。同时病程相关蛋白几丁质酶和β-1,3-葡聚糖酶的活性也增加。利用半定量RT-PCR方法检测防御反应基因表达,结果表明BTH诱导首先激发了植株PR-1等基因参与的水杨酸信号传导防御反应途径的发生,同时PDF1.2基因的上调表达说明BTH诱导也影响了茉莉酸信号传导途径。
译  名：
Studies on BTH-induced resistance to Sclerotinia sclerotiorum in cauliflower and physiology basis
作  者：
SUN Rong-rong1,PENG Zhen1,CHENG Lin1,ZHU Xiang-fei1,SHAO Tai-liang2,LU Gang1(1Department of Horticulture,College of Agriculture & Biotechnology,Zhejiang University,Hangzhou 310029,China;2 Zhejiang Sky Good Seed Co.Ltd,Wenzhou 325011,China)
关键词：
Brassica oleracea L.var.botrytis L.;Sclerotinia sclerotiorum Lid de Bary;BTH;induced disease resistance;gene expression
摘  要：
The effects of benzothiadiazole(BTH) spraying on cauliflower resistance to Sclerotinia sclerotiorum were evaluated.The disease indices were investigated in susceptible and resistant varieties inoculated with mycelium agar disks to seedling leaves.The results showed that BTH significantly reduced the size of rot tissue compared with controls,and the control efficacies of BTH treatment were 81.5% and 63.8% in susceptible-and resistant-varieties respectively.The activities of defensive enzymes such as POD,SOD,CAT,PAL,and PPO were increased in BTH-treated seedlings after inoculation with S.sclerotiorum.The activities of chitinase and β-1,3-glucanase in cauliflower also increased in BTH-treated seedlings.The expression patterns of two marker genes in defense signaling pathways and four pathogenesis-related protein genes were detected using semi-quantified RT-PCR.The results indicated that BTH-induced resistance to S.sclerotiorum in cauliflower mainly through SA-dependent signaling pathway,and the up-regulated expression of PDF1.2 showed JA/ET signaling pathway may also influenced by BTH-induced resistance in cauliflower.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46