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Position: Home > Articles > Study on the Commonality of Camellia sinensis EST-SSRs in Theaceae Plant Journal of Anhui Agricultural Sciences 2011,39 (13) 48-50

茶树EST-SSRs在山茶科植物中的通用性研究

作  者:
程小毛;陈自兰;王华
单  位:
湖北省农业科学院中药材研究所;西南林业大学园林学院
关键词:
茶树;EST-SSR;通用性;山茶科植物
摘  要:
[目的]研究茶树EST-SSRs在山茶科植物中的通用性。[方法]选用来源于茶树EST序列的7对EST-SSRs引物,对山茶科植物的19份材料进行了扩增。[结果]7对EST-SSRs引物中有5对引物能对19个供试材料进行有效扩增,通用性比率为71.43%;扩增率最高的为云南山茶凤山茶、华东山茶彩霞和云南山茶菊瓣,最低的为川滇莲蕊茶和大花杨桐;有效扩增的5对引物对中有4对引物在供试材料中表现出丰富且差异明显的多态性。[结论]从茶树基因组上开发的SSR引物在山茶科植物不同种属植物间有较高的通用性,可用于山茶科植物比较基因组研究和分析标记研究。
译  名:
Study on the Commonality of Camellia sinensis EST-SSRs in Theaceae Plant
作  者:
CHENG Xiao-mao et al(College of Landscape Architecture,Southwest Forestry University,Kunming,Yunnan 650224)
关键词:
Camellia sinensis;EST-SSR;Commonality;Theaceae plant
摘  要:
[Objective] The research aimed to study the commonality of Camellia sinensis EST-SSRs in Theaceae plant.[Method] Seven pairs of EST-SSRs primers which derived from Camellia sinensis EST sequence were used to amplify the nineteen species in Theaceae plant.[Result] Five pairs in the seven pairs of EST-SSRs primers could effectively amplify the nineteen test materials,and the commonality rate was 71.43%.The amplification rate of Camellia retiacalate Fengshancha,Camellia japouica Caixia and Camellia retiacalate Juban was the highest,and the amplification rate of Camellia synaptica Sealy and Adinandra sagonica var.Wallichiana(oc) Ming was the lowest.Moreover,four pairs in the five pairs of primers which could effectively amplify showed the rich polymorphism whose difference was obvious in the test materials.[Conclusion] SSR primers which were developed from the tea plant genome had the higher commonality in the different varieties of Theaceae plant and could be used in the comparative genome research and analysis mark research of Theaceae plant.

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