当前位置: 首页 > 文章 > DNA指纹技术在近交系小鼠遗传检测中的应用研究 甘肃农业大学学报 2009 (2) 32-35
Position: Home > Articles > Application of DNA fingerprinting in genetic monitoring of inbred strain mice Journal of Gansu Agricultural University 2009 (2) 32-35

DNA指纹技术在近交系小鼠遗传检测中的应用研究

作  者:
段天林;刘英;王芳;张文慧
单  位:
甘肃农业大学动物医学院;兰州大学医学实验中心
关键词:
近交系小鼠;DNA指纹技术;遗传检测
摘  要:
采用生物素标记的(GGAT)4寡核苷酸探针对C57BL/6J、BALB/c和DBA/2三种近交系小鼠进行了DNA指纹图分析.结果表明:(GGAT)4寡核苷酸探针对上述3种近交系小鼠产生的DNA指纹图的图带数均为10-12条,具有良好的多态性,品系内平均DNA指纹图的相似系数在0.92~1.00的范围内,具有相同指纹图的概率均在0.40以上,极显著地高于品系间的相似系数(0.21~0.39)和相同指纹图的概率(P<3.10×10-7),说明(GGAT)4寡核苷酸探针可用于制作近交系小鼠的DNA指纹图,以对其进行遗传检测.
译  名:
Application of DNA fingerprinting in genetic monitoring of inbred strain mice
作  者:
DUAN Tian-lin1,2,LIU Ying1,WANG Fang2,ZHANG Wen-hui2(1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;2.Center of Medical Laboratory,Lanzhou University,Lanzhou 730000,China)
关键词:
inbred strain mice;DNA fingerprinting;genetic monitoring
摘  要:
The objective of this paper is to evaluate DNA fingerprinting of BALB/c,C57BL/6J and DBA/2 inbred strain mice with the biotin-labeled(GGAT)4 oligonucleotide probe.There were 10-12 distinguishable bands in every DNA fingerprinting map and the oligonucleotide probe(GGAT)4 had an excellent polymorphism in 3 inbred strain mice.The similarity coefficient(x) within breeds was 0.92~1.00,and the probability of an identical DNA fingerprinting(P) was above 0.40.These two indices within breed were significantly higher than those between breeds(x=0.21~0.39,P<3.10×10-7).These results showed that the oligonucleotide probe(GGAT)4 can be used in DNA fingerprinting map for genetic monitoring of inbred strain mice.

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