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当前位置: 首页 > 文章 > 猪线粒体抗病毒信号蛋白MAVS对口蹄疫病毒感染宿主的影响 中国兽医学报 2017,37 (3) 393-397
Position: Home > Articles > MAVS with porcine mitochondrial antiviral signaling protein on host cell infected by FMD virus Chinese Journal of Veterinary Science 2017,37 (3) 393-397

猪线粒体抗病毒信号蛋白MAVS对口蹄疫病毒感染宿主的影响

作  者：
崔晓蕊;李守湖;秦晓东;常兴妮;孙鹏;孙继国;李志勇
单  位：
甘肃农业大学动物医学院;河北农业大学动物医学院;中国农业科学院兰州兽医研究所
关键词：
线粒体;MAVS;口蹄疫病毒;实时荧光定量PCR
摘  要：
采用构建猪源MAVS重组质粒pEGFP-MAVS,将其转染PK-15细胞24h后,再感染口蹄疫病毒(MOI=0.1),通过Western blot和real-time PCR检测MAVS融合蛋白表达及对口蹄疫病毒感染宿主细胞的复制和病毒感染滴度。结果显示:重组质粒pEGFP-MAVS能在PK-15细胞中获得表达,并且上调MAVS基因对口蹄疫病毒在宿主细胞中的早期复制有显著抑制作用,病毒的感染滴度也有一定的降低。结果表明:MAVS具有一定的抗病毒作用,此为进一步研究口蹄疫病毒逃逸宿主天然免疫的机制奠定了基础。
译  名：
MAVS with porcine mitochondrial antiviral signaling protein on host cell infected by FMD virus
作  者：
CUI Xiao-rui;LI Shou-hu;QIN Xiao-dong;CHANG Xing-ni;SUN Peng;SUN Ji-guo;LI Zhi-yong;College of Veterinary Medicine,Hebei Agricultural University;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences;College of Veterinary Medicine,Gansu Agricultural University;
关键词：
mitochondria;;MAVS;;foot and mouth disease virus;;real-time PCR
摘  要：
To study the impact of swine mitochondrial antiviral signaling protein MAVS on the infection of foot-and-mouth disease virus(FMDV)to host cells,recombinant plasmid swine MAVS pEGFP-MAVS was constructed,after transfecting PK-15 cells 24h,and then infected FMDV(MOI=0.1).Western blot and real-time PCR were used to detect expression of MAVS fusion protein and its replication of FMDV infection to host cell and virus titer.The results showed that the recombinant plasmid pEGFP-MAVS could be expressed in PK-15 cells.And the upregulation MAVS gene can significant inhibition the host cell early replication,and there was a little decrease in virus titer.The study showed that MAVS has some antiviral function,which provided basis for further research of FMDV escape mechanisms to host innate immune.

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