关键词：
R-28725;氯磺隆;GSH;ALS;GST
摘 要：
采用生物测定法研究了安全剂R-28725保护玉米免受除草剂氯磺隆药害的作用。在氯磺隆毒土浓度为2μg/kg时,使用5mg/kg R-28725浸种处理,玉米根部受到氯磺隆抑制的谷胱甘肽(GSH)含量由空白对照的57.3%恢复至83.4%;玉米叶片中乙酰乳酸合成酶(ALS)活性增加了17.2%;玉米根部的谷胱甘肽-S-转移酶(GST)活性增加了191.8%;GST酶促反应动力学参数Vmax增大102.5%,Km减小40.0%。结果显示:R-28725能够提高氯磺隆处理后玉米中GSH的含量,增加GST和ALS活性,增强了玉米根部GST酶对底物的亲和力,缓解氯磺隆对玉米产生的药害。
译 名：
The effects of the herbicide safener R-28725 in protecting maize from the phytotoxicity of the herbicide chlorsulfuron
作 者：
Zhao Lixia,Liu Chengguo,Fu Ying,Gao Shuang,Ye Fei(Northeast Agricultural University,Harbin 150030,China)
关键词：
R-28725;chlorsulfuron;GSH;ALS;GST
摘 要：
Physiological and biochemical tests were conducted to study the protective effects of the herbicide safener R-28725 in reducing the phytotoxicity of chlorsulfuron to maize.The GSH content in maize roots was increased from 57.3% to 83.4%,the ALS activity in shoots was increased by 17.2%,and the GST activity in roots was increased by 191.8%,when 2 μg/kg chlorsulfuron was used in soils and the seeds were soaked in 5 mg/kg R-28725.When treated only by R-28725,the kinetic parameters Vmax of GST was increased by 102.5%,and Km was decreased by 40.0%.The results suggested that R-28725 could improve the GSH content,GST activity,and ALS activity.The maize could be restored from the injury of chlorsulfuron by enhancing GST affinity for the CDNB substrate.
