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Position: Home > Articles > Comparsion of HI, GICA and AGID for Detecting Antibody against Avian Influenza Virus in Chickens China Animal Health Inspection 2006,23 (8) 23-24

三种检测禽流感抗体方法的比较

作  者:
冯娟;陈义平;王志亮;刘春菊;张喜悦
单  位:
中国动物卫生与流行病学中心
关键词:
禽流感抗体;胶体金免疫层析法;血凝抑制试验;琼脂扩散试验
摘  要:
应用胶体金免疫层析法(GICA)、血凝抑制试验(HI)和琼脂扩散试验(AGID)三种方法同时检测所采集的342份鸡血清样品,并对三者的检测结果进行比较。结果发现,HI试验的阳性检出率最高(78.65%),其次为GICA(69.88%)和AGID试验(59.36%);GICA与HI的符合率为89.47%,与AGID的符合率为84.21%,HI与AGID的符合率为80.70%。结果表明,GICA比AGID试验更为敏感,且与HI试验有较好的符合率,可用于禽流感的血清学监测。
译  名:
Comparsion of HI, GICA and AGID for Detecting Antibody against Avian Influenza Virus in Chickens
作  者:
Feng Juan1,2),Chen YiPing1),Wang ZhiLiang1),Liu ChunJu1), Zhang XiYue1) (1. China Animal Health&Epidemiology Center,Qing dao Shan dong 266032; 2. Veterinary College of Yangzhou University,YangZhou Jiangsu 225009)
关键词:
Avian Influenza Antibody; GICA; HI; AGID
摘  要:
253 chicken serum samples were collected to detect antibody against influenza A virus by hemagglutination inhibition (HI) test, gold immuno-chromatographic assay (GICA) and agar gel immunodiffusion (AGID) test. The results of the three assays were compared, and the positive ratio of HI test was 78.65%, GICA was 69.88%, AGID test was 59.36%, the correlation between GICA and HI, GICA and AGID, HI and AGID was 89.47% (306/342), 84.21% (288/342) and 80.70% (276/342) respectively. The results showed that GICA was specific and sensitive for the antibody detection of avian influenza A in chickens.

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