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当前位置: 首页 > 文章 > 天津地区规模化生猪场产肠毒素大肠杆菌的分离鉴定 天津农学院学报 2018 (3) 52-56
Position: Home > Articles > Isolation and identification of enterotoxigenic Escherichia coli from large scale pig farms in Tianjin area Journal of Tianjin Agricultural University 2018 (3) 52-56

天津地区规模化生猪场产肠毒素大肠杆菌的分离鉴定

作  者：
姜轩;刘梦月;付超;马吉飞;赵瑞利;石青青;胡烨;崔君
关键词：
产肠毒素大肠杆菌(ETEC);腹泻;分离鉴定;16S rRNA
摘  要：
具有特殊血清型的大肠杆菌对人与动物肠道有不同程度的致病性,其中以产肠毒素大肠杆菌(ETEC)危害较为严重。从天津地区规模化养猪场采集15例腹泻病猪的直肠拭子样品,接种到MH、麦康凯、伊红美蓝琼脂培养基进行细菌培养及革兰染色镜检。选取培养基上形态不同的菌落进行生化试验,16S rRNA序列测定及BLAST分析,结果分离到的10株肠杆菌科细菌与大肠杆菌的同源率达99.6%。合成大肠杆菌肠毒素ST1、ST2、LT1、IR1特异性引物,PCR结果检测到产LT1毒素、产LT1和ST1毒素及产ST1肠毒素的3株大肠杆菌。对分离出的产肠毒素LTI、ST1的菌株进行药敏试验,结果表明产肠毒素大肠杆菌对新霉素、克林霉素、庆大霉素、青霉素、头孢曲松、丁胺卡那、头孢呋辛、氟苯尼考敏感,为临床产肠毒素大肠杆菌的监测与防控提供依据。
译  名：
Isolation and identification of enterotoxigenic Escherichia coli from large scale pig farms in Tianjin area
作  者：
JIANG Xuan;LIU Meng-yue;FU Chao;MA Ji-fei;ZHAO Rui-li;SHI Qing-qing;HU Ye;CUI Jun;College of Animal Science and Veterinary Medicine, Tianjin Agricultural University;
单  位：
JIANG Xuan%LIU Meng-yue%FU Chao%MA Ji-fei%ZHAO Rui-li%SHI Qing-qing%HU Ye%CUI Jun%College of Animal Science and Veterinary Medicine, Tianjin Agricultural University
关键词：
enterotoxigenic Escherichia coli(ETEC);;diarrhea;;isolation and identification;;16S rRNA
摘  要：
Escherichia coli with special serotype has different degrees of pathogenicity to human and animal intestines, among which enterotoxigenic Escherichia coli(ETEC)is more serious. Rectal swabs samples of 15 diarrhoea pigs in Tianjin area were collected and inoculated with MH, MacConkey Agar and eosin-methylene blue agar for bacterial culture and Gram staining microscopy.The colonies with different shape on the medium were tested for biochemical tests, 16S rRNA sequencing and BLAST analysis. The homology rate of 10 strains of Enterobacteriaceae and Escherichia coli was 99.6%. The specific primers of Escherichia coli enterotoxin ST1, ST2, LT1 and IR1 were synthesized. PCR results showed that 3 strains of Escherichia coli producing LT1 toxin, LT1 and ST1 toxin, and ST1 enterotoxin were isolated. The antimicrobial susceptibility test of isolated strains of enterotoxin LTI and ST1 showed that enterotoxigenic Escherichia coli was sensitive to neomycin, clindamycin,gentamicin, penicillin, ceftriaxone, amikacin, cefuroxime and florfenicol, which provided a basis for the monitoring and prevention of ETEC.

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