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Position: Home > Articles > Study on Effects of Five Carbon to Decolor β-mannanase of Bacillus Subtilis FOOD SCIENCE 2003,24 (9) 106-108

活性炭对枯草芽孢杆菌β-甘露聚糖酶的作用

作  者:
余红英;孙远明;杨幼慧;杨跃生
单  位:
华南农业大学食品学院;华南农业大学生命科学院
关键词:
活性炭;β-甘露聚糖酶;纯化;脱色
摘  要:
考察了活性炭对枯草芽孢杆菌β-甘露聚糖酶液的脱色与纯化作用。结果表明,酶液在脱色温度50℃、活性炭量3%、吸附时间2h条件下,不仅可使酶液脱去一部分色素,并纯化酶3.77倍,收率达到86.19%±2.99%。
译  名:
Study on Effects of Five Carbon to Decolor β-mannanase of Bacillus Subtilis
作  者:
Yu Hongying et al.
关键词:
active carbon;β- mannanase;purification;decolorizing
摘  要:
The effect of active carbon on theβ- mannanase of Bacillus subtilis was investigated. The results showed that carbonwas found to be very effective for decolorizing crude beta-mannanase. The conditions of using 3% carbon, treated at 50℃ andpH 6.5 for a duration of 2h were optimal for decolorizing and purifying the enzyme. Under these conditions, the enzyme couldbe purified by 3.77±0.15 fold with a yielding recovery of 86.19%±2.99%.

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