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当前位置: 首页 > 文章 > 黑米通过上调小肠ABCG5/8和ABCA1基因表达降低胆固醇水平 食品科学 2015,36 (9) 136-140
Position: Home > Articles > Black Rice Reduces Cholesterol Level by Upregulating ABCG5/8 and ABCA1 mRNA Expression in Intestines FOOD SCIENCE 2015,36 (9) 136-140

黑米通过上调小肠ABCG5/8和ABCA1基因表达降低胆固醇水平

作  者：
马娜;申婷婷;赵江;王佳楠;张泽生
单  位：
天津科技大学食品工程与生物技术学院天津科技大学生物工程学院;天津科技大学食品工程与生物技术学院天津科技大学生物工程学院南开大学滨海学院;天津科技大学食品工程与生物技术学院
关键词：
黑米;小鼠;ABCG5/8;ABCA1;NPC1L1
摘  要：
以高脂膳食饲喂致高胆固醇小鼠为动物模型,研究黑米对小鼠血脂水平及小肠胆固醇代谢相关基因调控的影响。将48只雄性C57BL/6J小鼠随机分成高脂对照组和3个实验组(白米组、黑米低剂量组、黑米高剂量组),测定血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)及高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)水平,气相色谱法检测肝脏中胆固醇含量,实时荧光定量聚合酶链式反应(real-time polymerase chain reaction,real-time PCR)分析调控小肠胆固醇合成、吸收、转化及排泄基因HMG-Co A-R、MTP、ABCG5/8、ABCA1、NPC1L1等的m RNA表达水平。结果表明:与高脂对照组相比,白米组小鼠血清中TC、TG、HDL-C含量无统计学差异,但黑米低、高剂量组小鼠血清中TC和TG含量降低,且HDL-C含量显著或极显著升高(P<0.05或P<0.01)。与高脂对照组相比,白米组小鼠肝脏中胆固醇含量无显著变化,黑米低剂量组显著降低(P<0.05),黑米高剂量组极显著降低(P<0.01)。Real-time PCR结果显示,与高脂对照组相比,黑米低、高剂量组ABCG5/8、ABCA1 m RNA表达水平均极显著上调(P<0.01);黑米低剂量组NPC1L1 m RNA表达水平降低(P<0.05),黑米高剂量组极显著降低(P<0.01)。黑米对高脂膳食饲喂致高胆固醇小鼠胆固醇代谢平衡的调节可能是通过增加小肠中胆固醇的排泄并抑制其吸收实现的。
译  名：
Black Rice Reduces Cholesterol Level by Upregulating ABCG5/8 and ABCA1 mRNA Expression in Intestines
作  者：
MA Na;SHEN Tingting;ZHAO Jiang;WANG Jianan;ZHANG Zesheng;WANG Hao;School of Food Engineering and Biological Technology, Tianjin University of Science and Technology;College of Biotechnology, Tianjin University of Science and Technology;Binhai College, Nankai University;
关键词：
black rice;;mice;;ABCG5/8;;ABCA1;;NPC1L1
摘  要：
The influence of black rice on serum lipid(TC, TG, HDL-C) levels and the expression of intestinal cholesterol metabolism-related genes(HMG-Co A-R, MTP, ABCG5/8, ABCA1 and NPC1L1) induced by high fat and high cholesterol diet(HFHC) was investigated in mice. Male mice(n = 48) were divided into four groups. They were fed a HFHC diet alone(C group, n = 12), or supplemented with 52.6 g/100 g corn starch or with white rice(C+W group, n = 12), 26.3 g/100 g black rice(C+BL group, n = 12) or 52.6 g/100 g black rice(C+BH group, n = 12). After administration for 8 weeks, blood samples were collected for lipid measurements and viscera were removed at the end of the protocol. Serum total cholesterol(TC), triglyceride(TG) and high-density lipoprotein cholesterol(HDL-C) were assayed by commercial kits. Gas chromatography(GC) was used to determine cholesterol content in hepatic cells. The m RNA expression levels of intestinal HMG-Co A-R, MTP, ABCG5/8, ABCA1 and NPC1L1 were detected by real-time PCR. The contents of serum TC, TG and HDL-C in the experimental groups were similar with those of the control group. However, HDL-C in the black rice groups was significantly elevated when compared with that of the control group(P < 0.05 or P < 0.01). Hepatic cholesterol content were significantly decreased in the C + BL(P < 0.05)and C + BH(P < 0.01)groups when compared with that of the C group. The m RNA expression levels of ABCG5/8 and ABCA1 in intestines were up-regulated by dietary back rice(P < 0.01) when compared with those of the C group. The expression level of NPC1L1 m RNA was downregulated in the C + BL(P < 0.05) and C + BH(P < 0.01) groups. The increase of cholesterol excretion and the inhibition of cholesterol absorption in intestines may explain the mechanisms underlying the hypolipidemic effect of black rice.

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