译 名：
Effect of Niujiaodihuang Decoction on Drug Resistance and the Genetic Transcription of Staphylococcus aureus
作 者：
FENG Shuo;ZHANG Yafei;LI Yunpeng;YANG Zuojun;SUN Yingjian;College of Animal Science and Veterinary Medicine,Beijing University of Agriculture;
单 位：
FENG Shuo%ZHANG Yafei%LI Yunpeng%YANG Zuojun%SUN Yingjian%College of Animal Science and Veterinary Medicine,Beijing University of Agriculture
关键词：
Niujiaodihuang decoction;;Staphylococcus aureus;;drug resistance;;genomics analysis
摘 要：
【Objective】This study was aimed to evaluate the effect of Niujiaodihuang decoction(NDD) on the susceptibility of Staphylococcus aureus(S. aureus) against methicillin.【Method】The effect of NDD on the tolerance of S. aureusto oxacillin sodium was detected by microdilution method. The gene transcriptional changes of S. aureus were analyzed by gene transcriptional analysis,and the transcriptional changes of the related resistance genes were verified by PCR.【Result】The combined effect of NDD and oxacillin improved the inhibitory effect of oxacillin on the proliferation of S. aureus,while inhibiting the transcription level of femB,one of theresistance-related genes,as well as the cell wall synthesis related genes glmU and murG. However it had no significant effect to the transcription of the beta lactamase(pbpB) and its receptor(pbpBR).【Conclusion】NDD can increase the sensitivity of methicillin resistant S. aureus to oxacillin sodium. This effect may be related to the reduction of the expression of the gene femB,glmU and murG.
