Position: Home > Articles > Construction of Adeno-associated Virus Vector for Mouse prnp Gene Targeting
Acta Agriculturae Jiangxi
2008,20
(5)
82-85
腺相关病毒介导的小鼠prnp基因打靶载体构建
作 者：
王海鹰;万家余;聂代邦;高宏伟
单 位：
吉林大学和平校区畜牧兽医学院;军事医学科学院军事兽医研究所
关键词：
prnp;基因敲除;腺相关病毒载体;融合PCR
摘 要：
利用三重融合PCR方法构建了小鼠prnp基因敲除载体。提取129小鼠基因组,设计引物扩增打靶载体同源臂,把两同源臂与neo基因三元件PCR扩增成一条融合基因。融合基因与AAV载体酶切后的载体骨架通过NotI酶切位点相连接,构建重组质粒载体rAAV。
译 名：
Construction of Adeno-associated Virus Vector for Mouse prnp Gene Targeting
作 者：
WANG Hai-ying1,2,WAN Jia-yu1,NIE Dai-bang2,GAO Hong-wei1* (1.Institute of Military Veterinary,Academy of Military Medical Sciences,Changchun 130062,China; 2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China)
关键词：
prnp;Gene knockout;Adeno-associated virus vector;Fusion PCR
摘 要：
The adeno-associated virus vector for mouse prnp gene targeting was constructed by using three-way fusion PCR method.129 mouse genomes were extracted,the primer for amplification of homology arms was designed,and then the left homology arm,right homology arm and neo gene were fused to one gene.The recombination plasmid vector rAAV was constructed by ligating fusion gene into AAV vector backbone through NotI restriction enzyme site.
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