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Position: Home > Articles > Cloning of Some Genes Related to Cold-tolerance in Wheat Leaves Journal of Henan Agricultural Sciences 2009,38 (5) 20-23

小麦叶片内几个抗冷相关基因cDNA片段的克隆

作  者:
康国章;岳彩凤;沈丙权;刘超;王永华;郭天财
单  位:
河南农业大学国家小麦工程技术研究中心
关键词:
小麦;抗冷基因;克隆
摘  要:
运用RT-PCR法,从小麦叶片中克隆出SOD、CAT、APX、GR、CBF、GI等几个抗冷相关基因的cDNA片段,序列比对结果显示,这些基因与GenBank上已登录的大麦、小麦、玉米、水稻等相关基因的同源性较高,表明克隆出了上述几个小麦抗冷相关基因,其中的APX和GR为普通小麦中首次报道。
译  名:
Cloning of Some Genes Related to Cold-tolerance in Wheat Leaves
作  者:
KANG Guo-zhang,YUE Cai-feng,SHEN Bing-quan,LIU Chao,WANG Yong-hua,GUO Tian-cai* (Engineering Research Center for Wheat,Henan Agricultural University,Zhengzhou 450002,China)
关键词:
Triticum aestivum L.;Cold-tolerant genes;Cloning
摘  要:
Partial cDNA sequences of superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX),glutathione reductase(GR),C-repeat bing factor(CBF) and GIGANTEN(GI) were isolated from wheat leaves using RT-PCR.Sequence analysis showed that these genes had high identity to relevant genes from wheat,barley,maize and rice registered in GenBank,im-plying that these cloned fragments were really the cold-tolerant genes.Among them,APX and GR were firstly isolated from common wheat(Triticum aestivum L.).

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