当前位置: 首页 > 文章 > 苹果实生树阶段转变过程中丝氨酸羟甲基转移酶的变化 江西农业大学学报 2011,33 (2) 254-257+311
Position: Home > Articles > Changes of Serine Hydroxymethyltransferase during Phase Change in Apple(Malus domestica Borhk.) Acta Agriculturae Universitatis Jiangxiensis 2011,33 (2) 254-257+311

苹果实生树阶段转变过程中丝氨酸羟甲基转移酶的变化

作  者:
曾广娟;张新忠;李春敏
单  位:
河北省农林科学院昌黎果树研究所;中国环境管理干部学院;中国农业大学园艺植物研究所
关键词:
苹果;阶段转变;丝氨酸羟甲基转移酶;酶联免疫(ELISA);免疫组织化学(IHC)
摘  要:
用酶联免疫(ELISA)法和免疫组织化学方法(IHC)检测5年生苹果实生树不同节位的丝氨酸羟甲基转移酶(SHMT)的变化。结果表明,此酶只存在苹果实生树营养生长期的叶片中,而在童期的叶片和生殖生长期的叶片中含量极低或检测不到。SHMT在苹果实生树阶段转变过程中存在差异表达。
译  名:
Changes of Serine Hydroxymethyltransferase during Phase Change in Apple(Malus domestica Borhk.)
作  者:
ZENG Guang-juan1,2,ZHANG Xin-zhong3,LI Chun-min2 (1.Environmental Management College of China,Qinhuangdao 066004,China;2.Changli Institute of Pomology,Hebei Academy of Agricultural and Forestry Science,Changli 066600,China;3.Institute for Horticultural Plants,China Agricultural University,Beijing 100193,China)
关键词:
Malus domestica Borhk.;phase change;SHMT;ELISA;IHC
摘  要:
Changes of serine hydroxy methyl transferase(SHMT) in leaves from different nodes of five-year-old apple seedlings were analyzed with enzyme-linked immunosorbent assay(ELISA) and immunocytochemistry(IHC)methods.The results showed that SHMT was only prestent in leaves of vegetative phase and was absent in the same organ of juvenile and reproductive phase.

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