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Position: Home > Articles > Analysis of Curcumin by High Performance Thin-Layer Chromatoraphy(HPTLC) FOOD SCIENCE 2004,25 (6) 156-159

姜黄素的高效薄层色谱分析

作  者:
徐仲溪;王坤波
单  位:
湖南农业大学食品科技学院;湖南省天然产物工程技术研究中心
关键词:
薄层色谱扫描法;姜黄;姜黄素;薄层扫描
摘  要:
以硅胶GF254板为固定相、氯仿-甲醇-甲酸(体积比为96:4:0.7)为流动相,建立了姜黄素薄层色谱分析方法。姜黄素样品溶液上行展开5.0cm,其Rf值为0.70。姜黄素的检测量在27~216ng范围内,其斑点的面积与姜黄素的检测量具有良好的线性关系,相关系数为0.9983。姜黄素的平均回收率为102.08%,方法的精密度良好(RSD≤1.5%)。该方法简便、快速、准确,可用于姜黄样品的定性定量分析。
译  名:
Analysis of Curcumin by High Performance Thin-Layer Chromatoraphy(HPTLC)
作  者:
XU Zhong-xi1,WANG Kun-bo2(1.College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China;2.Hunan Provincial Engineering Research Center for Natural Products,Changsha 410128,China)
关键词:
thin-layer chromatography;curcuma Longa;curcumin;thin-layer scan
摘  要:
The thin-layer chromatographic method of curcumin was developed by the use of chloroform-methanol-formic acid(96:4:0.7 in volume ratio) as a mobile phase and on GF254 silica gel plate. The Rf value of cuecumin was 0.70. The calibration curvewas linear (r=0.9983) in the range of 27~216ng of curcumin and the average recovery of 102.08%.The results showed it to bea simple, rapid sensitive and reproducible method for the determination of curcumin in curcuma samples and curcumin extract.

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