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Acta Horticulturae Sinica
1999,26
(2)
利用Lfy cDNA转化猕猴桃的研究
作 者：
郭卫东;沈向;李嘉瑞;郑学勤
单 位：
中国热带农业科学院热带作物生物技术国家重点实验室;西北农业大学园艺系
关键词：
猕猴桃；Lfy cDNA；遗传转化
摘 要：
利用促进早实性状的ＬｆｙｃＤＮＡ转化猕猴桃，建立了猕猴桃遗传转化体系。经过点杂交检测初步证实，采用农杆菌菌株ＬＢＡ４４０４介导的叶盘法转化猕猴桃，将ＬｆｙｃＤＮＡ导入猕猴桃基因组中。
译 名：
Agrobacterium Tumefaciens Mediated Transformation of Kiwifruit with Lfy cDNA
作 者：
Guo Weidong1,Shen Xiang1,Li Jiarui1,and Zheng Xueqin2 (1Department of Horticulture Northwestern,Agricultural University,Yangling,Shaanxi 712100; 2National Key Biotechnology Laboratory for Tropical Crops,CATAS,Danzhou,Hainan 571737)
关键词：
Kiwifruit;Lfy cDNA;Genetic transformation
摘 要：
Genetic transformation system for kiwifruit was set up through transformation of kiwifruit with Lfy cDNA which is useful for enhancing early fruit set.The putative transformants was obtained by cocultivation of kiwileafdiscs with Agrobacterium tumefaciens strain LBA4404.Transgenic lines intigrating Lfy cDNA were certified by dot blotting.
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