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当前位置: 首页 > 文章 > 猪圆环病毒II型PCR检测方法的研究 中国农学通报 2007,23 (9) 28-31
Position: Home > Articles > Detection of Procine Circovirus Type 2 by PCR Technique Chinese Agricultural Science Bulletin 2007,23 (9) 28-31

猪圆环病毒II型PCR检测方法的研究

作  者：
王宪文;刘兴友;梁美兰;郑玉姝;刘丽艳
单  位：
河南科技学院动物科学学院
关键词：
猪圆环病毒II型;PCR;检测
摘  要：
【目的】建立快速、简便、特异的检测猪圆环病毒II型的PCR方法;【方法】根据已发表的猪圆环病毒II型基因序列设计合成了一对引物,用酚-氯仿法抽提可疑病料中的病毒DNA,应用PCR技术对猪圆环病毒进行基因扩增,PCR产物进行序列测定;以猪瘟病毒、猪伪狂犬病毒、猪细小病毒为对照,进行特异性试验;取部分病料进行重复性试验;【结果】PCR方法扩增出了长度为702bp的片段,扩增产物经测序和序列分析表明扩增的序列为PCV2序列;猪瘟病毒、猪伪狂犬病毒、猪细小病毒的PCR扩增均为阴性,与预期结果一致;部分病料PCR重复检测5次,检测结果完全一致;【结论】PCR检测方法可以对猪圆环病毒病作出敏感、特异、准确地诊断,该方法检测的基因最低模板量为2×10-5ng/ml。
译  名：
Detection of Procine Circovirus Type 2 by PCR Technique
作  者：
Wang Xianwen,Liu Xingyou,Liang Meilan,Zheng Yushu,Liu Liyan(College of Animal Science,Henan Institute of Science and Technology,Henan,Xinxiang 453003)
关键词：
PCV2,PCR,Detection
摘  要：
[Objective]A polymerase chain reaction for detecting PCV2 was established;[Mothed]Based on the gene sequence of PCV2,a pair of primers was designed.viral DNA from the sick tissues suspious of inclouding PCV2 extracted with phenol/chloroform as template,PCR technique was used;the special test of PCR detection was taken with the Hog choleravirus,Pseudorabies virus and Swine Parvovirus virus as control;the repetitive test of PCR detection was also taken.[Result]A size of 702 bp was amplified by PCR,and the PCR production was sequenced,sequence analysis showed that the sequence belonged to the genome of PCV2;the Hog choleravirus,Swine Pseudorabies virus and Swine Parvovirus virus were also detected by the PCR as control under the same condation with negative results;some sick tissues were detected for 5 times with high repetitive results.[Conclusion]The method of PCR detection was a sensitive,specific and reliable method for detecting PCV2,the sensitivity of PCR detection for PCV2 was up to 2×10-5ng/ml DNA of positive cell virus infected PCV2.

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