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当前位置: 首页 > 文章 > 4株沙漠土壤衣藻的分离培养及形态和分子鉴定 石河子大学学报(自然科学版) 2013,31 (3) 294-300
Position: Home > Articles > Study on Morphology and Molecular Phylogeny of Four Desert Chlamydomonas Journal of Shihezi University(Natural Science) 2013,31 (3) 294-300

4株沙漠土壤衣藻的分离培养及形态和分子鉴定

作  者：
龚春霞;王丹;苟亚峰;左晓旭;李芳芳;高剑峰
单  位：
石河子大学生命科学学院
关键词：
古尔班通古特沙漠;塔克拉玛干沙漠;衣藻;18SrDNA
摘  要：
从古尔班通古特沙漠和塔克拉玛干沙漠采集沙样,先进行混合培养,混培藻液采用96孔板有限稀释法和平板法,分离到4株微藻(GTD2A,GTD4C,TLD7A-2,3a-5),采用传统形态鉴定方法及18SrDNA片段克隆分别对筛选的4株微藻进行鉴定,并进行系统发育分析。结果表明:通过形态学特征将4株藻初步鉴定为绿藻门、绿藻纲、团藻目、衣藻科。采用分子生物学方法克隆的18SrDNA片段进行复核的结果表明GTD2A2与GenBank数据库中的Chlamydomonas debaryana(FR865608.1)同源性达到了99.92%,说明已经鉴定至种。GTD3a-5、GTD4C、TLD7a-2与GenBank数据库中Chlamydomonas baca(FR865613.1)、C.reinhardtii和Chlamydomonas sp.(FR865563.1)3个藻种的同源性分别达到了98.10%、98.69%和97.65%。研究确定了4株沙漠衣藻的分类地位及其同其它衣藻种的亲源关系,为以后的研究沙漠藻的分子生物学和生理学研究奠定了基础。
译  名：
Study on Morphology and Molecular Phylogeny of Four Desert Chlamydomonas
作  者：
GONG Chunxia,WANG Dan,GOU Yafeng,ZUO Xiaoxu,LI Fangfang,GAO Jianfeng (College of Life Science,Shihezi University,Shihezi 832000,China)
关键词：
Gurbantunggut Desert;Takilimakan Desert;chlamydomonas;18SrDNA
摘  要：
To study microalgae species of Gurbantunggut Desert and Takilimakan Desert,this experiment isolated four desert microalgae from the sand samples by using the method of 96-well plate limited dilution and spread plate method,and studied the morphology and phylogeny in a polyphasic approach.Morphologically,the investigated strains could be discriminated in Chlorophyta,Volvocales,Chlamydomonadaceae.The morphological differences were reflected in the four distinct species.The GTD2A2 strain had very high similarity of 99.92% to Chlamydomonas debaryana (FR865608.1), which demonstrated that GTD2A2 strains were identified to species.The 18SrDNA gene sequences of GTD3a-5,GTD4C,and TLD7a-2strain were 98.10%,98. 69% and 97.65% similar to Chlamydomonas baca (FR865613.1), C.reinhardtii (M32703.1) and Chlamydomonas sp. (FR865563.1), which demonstrated that GTD3a-5,GTD4C,GTD7A-2strains were identified to genus.This study clearly defines the taxonomic status of 4strains of desert Chlamydomonas and Chlamydomonas with other relatives,providing a valuable foundation for further study of desert algae in the molecular biology and physiology.

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