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当前位置: 首页 > 文章 > 猪伪狂犬病油乳剂灭活疫苗的制备及安全性与免疫性试验 畜牧兽医学报 2001,32 (1) 44-51
Position: Home > Articles > THE PREPARATION OF SWINE PSEUDORABIES OIL EMULSION INACTIVATED VACCINE AND EXPERIMENTS ON ITS SAFETY AND IMMUNITY Chinese Journal of Animal and Veterinary Sciences 2001,32 (1) 44-51

猪伪狂犬病油乳剂灭活疫苗的制备及安全性与免疫性试验

作  者：
陈焕春;金梅林;何启盖;方六荣;吴美洲;周斌;周锐
单  位：
华中农业大学牧医学院
关键词：
猪伪狂犬病;灭活疫苗;微量中和试验;安全性;免疫性
摘  要：
用本室分离鉴定的猪伪狂犬病毒鄂A强毒株接种仓鼠肾细胞 (BHK 2 1) ,制备病毒抗原液 ,毒价不低于 10 6TCID50 / 0 1ml。经一定浓度的甲醛溶液灭活后与油相佐剂乳化研制成油乳剂灭活油疫苗 4批。本研究对该制品的安全性、免疫性进行了测定。对 18g左右小白鼠接种 0 3ml,初生仔猪、断奶仔猪及妊娠母猪加倍剂量注射 ,均未出现不良反应 ,安全性良好。对母猪的繁殖性能不产生影响。后备母猪及妊娠母猪血清中和抗体指数于免疫后 2 1d达到 316以上 ,间隔 35d加强免疫一次后 ,中和抗体指数可达 10 0 0以上。断奶仔猪及初生仔猪免疫后对强毒的攻击 ,保护率分别为 10 0 %及 90 62 %。
译  名：
THE PREPARATION OF SWINE PSEUDORABIES OIL EMULSION INACTIVATED VACCINE AND EXPERIMENTS ON ITS SAFETY AND IMMUNITY
作  者：
Chen Huanchun,Jin Meilin,He Qigai,Fang Liurong, Wu Meizhou,Zhou Bin,Zhou Rui (Animal Science and Veterinary Medicine College, Huazhong Agricultural University,Wuhan 430070)
关键词：
Swine pseudorabies;Inactived vaccine;Safety;lmmunity;Micro neutralization test
摘  要：
The virulent pseudorabies virus Ea strain which was isolated and identified by our lab,was cultivated on BHK 21 cell and used as vaccine antigen,The virus titer was over 10 6 TCID 50 /0 1 ml The vaccines were prepared four times following the procedure;i e,inactivation of virus with a certain concentration of Formal in and then emulsion of the killed virus with oil adjuvant In this paper the safety and immunity of the inactivated vaccine were reported The 18 gram weight mice were injected with 0 3 ml of vaccine,while newborn piglets,post weaning piglets and pregnant sows accepted as much two fold as normal dosage The fact that no any reverse reaction was observed and the sow′s production was not affected revealed the high safety of the vaccine The serum neutralization index of replacement gilts and pregnant sows were over 316 at 21st days post immunization At interval of 35 days the sows accepted another injection,the SN index could raise to over 10 000 After challenge with virulent pseudorabies virus,the protective rate of immunized post weaning and newborn piglets were 100% and 90 62%,respectively

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