当前位置: 首页 > 文章 > 中性蛋白酶酶解蛋清制备活性肽的研究 安徽农业科学 2007,35 (11) 126-127
Position: Home > Articles > Study on Bioactive Peptide Preparation from Egg White by Neutral Protease Hydrolysis Journal of Anhui Agricultural Sciences 2007,35 (11) 126-127

中性蛋白酶酶解蛋清制备活性肽的研究

作  者:
金嫘;李新华
单  位:
沈阳农业大学食品学院
关键词:
蛋清;中性蛋白酶;活性肽;水解度
摘  要:
研究了AS1398中性蛋白酶酶解鸡蛋蛋清制备小分子活性肽,确定了酶解的最佳工艺:pH值8.0,酶解温度60℃,底物浓度4%,酶加入量为底物浓度的7%,水解时间4 h。在此条件下,水解度达到26.1%。用SephadexG-15测定了水解物分子量分布,结果表明:水解产物中的主要成分是分子量集中在1 300 Da的寡肽。
译  名:
Study on Bioactive Peptide Preparation from Egg White by Neutral Protease Hydrolysis
作  者:
JIN Lei et al(College of Food Science,Shenyang Agricultural University,Shenyang,Liaoning 110161)
关键词:
Egg white;Neutral protease;Activity peptide;Hydrolysis degree
摘  要:
Bioactive peptide was prepared by AS1398 neutral protease hydrolysis from egg white.The optimum parameters were 8.0 pH,60 ℃ reaction temperature,4 % substrate concentration,7 % enzyme concentration of sabstrate for hydrolysis 4 h.Under this condition the hydrolysis degree reached 26.1 %.Molecular weight of peptide was determined by Sephadex G-15.The result showed that molecular weight of most peptides was about 1300Da.

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