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Position: Home > Articles > A Study on Isolation Identification and Drug Sensitivity Test of Chicken-derived Pathogenic Escherichia Coli Journal of Xichang University(Natural Science Edition) 2019 (1) 22-27

鸡源致病性大肠杆菌的分离鉴定及药敏试验

作  者:
任丽;徐睿;唐强;何欣怡
单  位:
西昌学院动物科学学院;攀西动物疫病检测与防控高校重点实验室
关键词:
鸡源大肠杆菌;分离鉴定;致病性;药敏试验
摘  要:
为了解鸡源致病性大肠杆菌的生物学特性和耐药性,试验通过采集病料、细菌分离培养、16S rRNA基因鉴定、致病性试验、药敏试验等方法。结果表明:分离出的20株细菌的培养特征、镜检特征均符合大肠杆菌特征;16S rRNA基因序列经BLAST比对与大肠杆菌同源性达99.9%~100%,确定20株分离菌为鸡源大肠杆菌;20株大肠杆菌的致病力为40%~100%;10株鸡源致病性大肠杆菌对15种抗菌药物均呈现多重耐药,耐药率为33.3%~93.3%,耐药情况较严重和复杂。
译  名:
A Study on Isolation Identification and Drug Sensitivity Test of Chicken-derived Pathogenic Escherichia Coli
作  者:
REN Li;XU Rui;TANG Qiang;HE Xinyi;School of Animal Science, Xichang University;Panxi Regional Key Laboratory of Animal Disease Detection and Prevention;
关键词:
chicken-derived Escherichia coli;;isolation and identification;;pathogenicity;;drug sensitivity test
摘  要:
To understand the biological traits and drug resistance of pathogenic Escherichia coli from chicken, we conducted the study by collecting disease materials, bacterial isolation and culture, 16 S rRNA gene identification,pathogenicity test, drug sensitivity test and other tests. The results show that the culture and microscopic traits of the20 isolates are consistent with the traits of E. coli; the 16 S rRNA gene sequence was 99.9%-100% homologous to E.coli by BLAST, and 20 isolates were identified as chicken-derived Escherichia coli; 20 strains of Escherichia coli have a pathogenicity of 40% to 100%; 10 strains of chicken-derived pathogenic Escherichia coli have multi-drug resistance to 15 antimicrobial agents, and the resistance rate is 33.3%-93.3%. The drug-resistant situation is serious and complicated.

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