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当前位置: 首页 > 文章 > 磷对成骨细胞超微结构及其GJIC和Ⅰ型胶原含量的影响 中国兽医科学 2011 (8) 839-843
Position: Home > Articles > Effects of phosphorus on ultramicrostructure,GJIC and content of collagen Ⅰ of osteoblasts in vitro Chinese Veterinary Science 2011 (8) 839-843

磷对成骨细胞超微结构及其GJIC和Ⅰ型胶原含量的影响

作  者：
卓丽玲;刘宗平
单  位：
扬州大学兽医学院;枣庄学院生命科学系
关键词：
成骨细胞;磷;超微结构;细胞间隙连接通讯
摘  要：
在体外培养成骨细胞(OB)的基础上,添加0、1、2、4mmol/L磷,作用2d后,采用电子显微镜观察OB的超微结构,采用划痕标记染料示踪技术观察细胞间隙连接通讯(GJIC);作用2、5、8d后,用BCA法蛋白定量试剂盒、Goat Anti-rat Collagen TypeⅠ试剂盒分别检测OB内总蛋白及Ⅰ型胶原的含量,并用DⅠ型胶原/D总蛋白表示Ⅰ型胶原的含量。结果显示,作用2d,4mmol/L磷组OB内线粒体减少、肿胀。4mmol/L磷组GJIC的荧光扩散距离减弱,1、2mmol/L磷组基本不变;就Ⅰ型胶原含量而言,除1mmol/L磷组在作用2d抑制其分泌外(P<0.05),在磷作用2、5、8d均促进其分泌(P<0.05)。结果表明,添加1、2、4mmol/L磷能促进OB的分泌Ⅰ型胶原;4mmol/L磷能抑制OB的代谢活性,且能抑制OB间通讯。
译  名：
Effects of phosphorus on ultramicrostructure,GJIC and content of collagen Ⅰ of osteoblasts in vitro
作  者：
ZHUO Li-ling1,2,LIU Zong-ping2 (1.Department of Life Science,Zaozhuang College,Zaozhuang 277160,China;2.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China)
关键词：
osteoblast;phosphorus;ultramicrostructure;gap junction intercellular communication(GJIC)
摘  要：
Osteoblasts(OB) were exposed to 0,1,2 and 4 mmol/L phosphorus in vitro to observe the ultramicrostructure by electronmicroscopy,and to observe the gap junction intercellular communication(GJIC) as well using nick marker dye tracer technology on day 2 post-exposure,and to detect contents of the total protein and collagen typeⅠ in OB on day 2,5 and 8 post-exposure using BCA method kit and Goat Anti-rat Collagen typeⅠ kit,respectively. The content of collagen type Ⅰ was expressed by Dcollagen type Ⅰ/Dtotal protein. In result,the mitochondria in 4 mmol/L P group were decreased on 2 day post-exposure. The fluorescence diffusion length of GJIC in 4 mmol/L P group was decreased and in 1 and 2 mmol/L P groups were unchanged on day 2 post-exposure. The contents of collagen type Ⅰ in 1,2 and 4 mmol/L P groups were all enhanced on day 2,5 and 8 post-exposure respectively(P<0.05) except 1 mmol/L P groups on day 2 post-exposure. In conclusion,P in the all exposure groups enhanced OB to secrete collagen type Ⅰ,and 4 mmol/L P inhibited metabolic activity and intercellular communication of OB.

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