中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 食源性乳杆菌中耐药基因的转移研究 食品科学 2015,36 (23) 167-171
Position: Home > Articles > Antimicrobial Resistant Gene Transfer of Foodborne Lactobacilli FOOD SCIENCE 2015,36 (23) 167-171

食源性乳杆菌中耐药基因的转移研究

作  者：
马沁沁;付雨;孙群
单  位：
四川大学生命科学学院
关键词：
耐药性;乳杆菌;基因转移;接合子;食品安全
摘  要：
为评估食品中乳杆菌(Lactobacillus spp.)耐药基因转移所引起的食品安全风险,对耐药乳杆菌菌株进行了红霉素、万古霉素和氟喹诺酮类药物耐药基因的聚合酶链式反应(polymerase chain reaction,PCR)检测,植物乳杆菌(Lactobacillus plantarum)、嗜酸乳杆菌(Lactobacillus acidophilus)和德氏乳杆菌(Lactobacillus delbrueckii)中检测到红霉素耐药基因msr C,万古霉素和氟喹诺酮类药物耐药基因检测结果为阴性。以供体菌和受体菌菌液体积比为10∶1,通过滤膜杂交法,进行乳杆菌耐药基因msr C对金黄色葡萄球菌(Staphylococcus aureus)的体外转移,但仅获得1个接合子菌落;乳杆菌对粪肠球菌(Enterococcus faecalis)msr C基因体外转移频率约为2.2×10-2,与敏感菌相比,接合子生长速率减缓。在无特定病原体(specific pathogen free,SPF)裸鼠肠道内进行的L.delbrueckii对E.faecalis的msr C基因体内转移中未检测到接合子。结果表明,乳杆菌携带的可转移耐药基因在肠道内传递至致病菌或机会致病菌的机率很小,且接合子生长比敏感菌缓慢,在抗生素选择压降低或消失的条件下易被淘汰。因此,目前食源性乳杆菌中耐药基因可能引起的食品安全风险较小。
译  名：
Antimicrobial Resistant Gene Transfer of Foodborne Lactobacilli
作  者：
MA Qinqin;FU Yu;SUN Qun;Key Laboratory of Bio-resource and Eco-environment,Ministry of Education,College of Life Sciences,Sichuan University;College of Life Sciences,Sichuan Normal University;
关键词：
antimicrobial resistance;;Lactobacillus;;gene transfer;;exconjugant;;food safety
摘  要：
To assess the risk of antimicrobial resistant gene transfer from foodborne Lactobacillus strains to human pathogens or opportunistic pathogens, we performed the detection of the resistant genes of erythromycin, vancomycin and fluoroquinolones in resistant Lactobacillus strains. Erythromycin resistant gene msr C was shown in the plasmids of Lactobacillus plantarum, Lactobacillus acidophilus and Lactobacillus delbrueckii, while negative results were obtained for vancomycin and fluoroquinol ones. Filter mating was carried out at a donor/recipient ratio of 10:1 with Staphylococcus aureus and E. faecalis as the recipients and L. delbrueckii the donors, respectively. In L. delbrueckii and S. aureus mating pair, only one exconjugant was obtained, and the average gene transfer frequency of L. delbrueckii to E. faecalis was 2.2 × 10-2, indicating that the mobile resistant gene from lactobacilli could disseminate to pathogens. The growth rate of exconjugants was lower than the control, so exconjugants might be washed out more easily than their susceptible counterpart when antimicrobial selective pressure was absent. The transfer of msr C from L. delbrueckii toE. faecalis was not observed in gnotobiotic rats. Accordingly, lactobacilli widely used in foods now may not increase the antimicrobial resistance in pathogens, thus threatening public health severely.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46