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Position: Home > Articles > Tissue Culture of Zhangzhou Narcissus Journal of Tropical Biology 2014,5 (4) 363-367

漳州水仙的组织培养技术

作  者:
金雄霞;詹富钧;罗越华;夏志辉
单  位:
海南大学热带生物资源可持续利用省部共建国家重点实验室培育基地;海南大学农学院
关键词:
漳州水仙;组织培养;植株再生
摘  要:
以漳州水仙为材料,研究了漳州水仙的组织培养技术,优选出不定芽再生与生根的最佳培养基,结果表明:鳞茎盘与愈伤组织分化不定芽的最优培养基都为MS+6-BA 10 mg·L-1+NAA 1.0 mg·L-1+蔗糖30 g·L-1+植物凝胶3 g·L-1;生根的最佳培养基为1/2 MS+NAA 0.1 mg·L-1+IBA 0.1mg·L-1+蔗糖15 g·L-1的液体培养基。
译  名:
Tissue Culture of Zhangzhou Narcissus
作  者:
JIN Xiongxia;ZHAN Fujun;LUO Yuehua;XIA Zhihui;Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources,Hainan University;College of Agronomy,Hainan University;
关键词:
Zhangzhou Narcissus;;tissue culture;;plant regeneration
摘  要:
Zhangzhou Narcissus( Narcissus tazetta var. chinensis),one of the 10 famous traditional flowers in China,was tissue cultured with bulb-scale and leaf segments as explants on the callus inducing and rooting mediums supplemented with different auxin combinations. Calli were induced from both segments on the mediums but no adventitious buds were developed from callus culture of bulb leaf segment. The optimal medium for adventitious bud formation from calli induced from bulb scale segments was MS + 6-BA 10 mg·L- 1+ NAA 1. 0mg·L- 1+ sucrose 30 g·L- 1+ phytagel 3 g·L- 1,and the optimal rooting medium was 1 /2 MS + NAA 0. 1 mg·L- 1+ IBA 0. 1 mg·L- 1+ sucrose30 g·L- 1+ phytagel 0 g·L- 1.

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