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当前位置: 首页 > 文章 > 猪瘟疫苗冻干保护剂的筛选及免疫效果评价 上海农业学报 2016,32 (2) 19-23
Position: Home > Articles > Screening of freeze-drying protectants of swine fever virus vaccine and effect evaluation for oral immunization Acta Agriculturae Shanghai 2016,32 (2) 19-23

猪瘟疫苗冻干保护剂的筛选及免疫效果评价

作  者：
李星;陶洁;殷秀辰;张清真;熊年年;刘惠莉
单  位：
上海海洋大学水产与生命学院;上海市农业科学院畜牧兽医研究所
关键词：
猪瘟疫苗;黏膜佐剂LTRG蛋白;冻干保护剂;口服免疫;效果评价
摘  要：
为了筛选耐热性能好、能提高猪瘟病毒(SFV)疫苗经口服途径免疫效果的冻干保护剂,配比不同组分的冻干保护剂与黏膜佐剂LTRG蛋白、SFV按比例混合,通过对冻干工艺的优化,制备了4种.SFV的冻干苗;口服免疫小鼠,测定血清IgG和粘膜IgA抗体效价。结果表明:试验2组即甘氨酸+明胶组和试验4组即明胶组的外观形态较好,水溶后呈现澄清的状态;试验2组和试验4组的冻干苗经口服免疫,诱导小鼠产生的IgG抗体水平显著高于SFV组、SFV+LT组及其他试验组(P<0.01)。二免7 d试验2组小鼠肛试子IgA抗体显著高于其他组(P<0.01),且小鼠脾脏IFN-γ和IL-4细胞因子的表达量也极显著高于其他组(P<0.01)。研究初步筛选出第2组配方,即以甘氨酸和明胶配伍作为猪瘟口服疫苗冻干保护剂,为研制口服途径免疫的猪瘟疫苗奠定了基础。
译  名：
Screening of freeze-drying protectants of swine fever virus vaccine and effect evaluation for oral immunization
作  者：
LI Xing;TAO Jie;YIN Xiu-chen;ZHANG Qing-zhen;XIONG Nian-nian;LIU Hui-li;College of Fisheries and Life Science,Shanghai Ocean University;Animal Husbandry and Veterinary Research Institute,Shanghai Academy of Agricultural Sciences;Shanghai Key Laboratory of Agricultural Genetics and Breeding;
关键词：
Swine fever virus vaccine;;Mucosal adjuvant LTRG protein;;Freeze-drying protectant;;Oral immunization;;Effect evaluation
摘  要：
In order to select a freeze-drying protectant having a good heat tolerance and improving oral immunization of swine fever virus(SFV) vaccine,freeze-drying protectants with different components were mixed with mucosal adjuvant LTRG protein and SFV in proportion,and 4 kinds of lyophilized SFV vaccines were prepared via optimization of freeze-drying conditions;The vaccines were used for oral immunization of mice and the serum IgG and mucosal IgA antibody valences were determined.The results showed that Group 2(glycine + gelatin) and Group 4(gelatin) had a relatively good appearance and water solubility;After oral immunization the mice-produced IgG antibody levels in Groups 2 and 4 were very significantly higher than those in other groups,and at 7 days after the second oral immunization the anal swab's IgA antibody level in Group 2 was very significantly higher than that in other groups,and moreover,the spleens'IFN-γ and IL-4 cytokine expression levels in Group 2 were very significantly higher than those in other groups.It was concluded that gelatin and glycine could be used as freeze-drying protectants of oral SFV vaccine.

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