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Position: Home > Articles > Amplifying and Homology Comparison of Heat Shock Protein 70 Gene in Pigeon Journal of Anhui Agricultural Sciences 2010,38 (36) 20712-20713+20716

鸽热休克蛋白70基因的扩增与同源性比较

作  者:
薛茂云;赵宝华;吕玲;刘萍;高琴;戴鼎震
单  位:
金陵科技学院;江苏经贸职业技术学院;中国农业科学院家禽研究所
关键词:
肉鸽;热休克蛋白70;RT-PCR;同源性
摘  要:
[目的]扩增鸽热休克蛋白(HSP)70基因,并与其他禽类作同源性比较。[方法]参考禽类的HSP70基因序列设计、合成引物,从热应激鸽肝脏组织中提取总RNA作为模板,RT-PCR扩增目的基因。[结果]RT-PCR扩增到的基因片段为576 bp,经测序,确定为鸽HSP70基因;鸽HSP70基因与鸡、鹌鹑、珍珠鸡同类基因序列的同源性达97%以上。[结论]禽类的HSP70基因具有较高保守性。
译  名:
Amplifying and Homology Comparison of Heat Shock Protein 70 Gene in Pigeon
作  者:
XUE Mao-yun et al (Jiangsu Institute of Economic and Trade Technology,Nanjing,Jiangsu 211196)
关键词:
Pigeon;Heat shock protein70;RT-PCR;Homology
摘  要:
[Objective] To amplify and do homology comparison of heat shock protein(HSP)70 gene in pigeon.[Method] A pair of primers were designed and synthesized using HSP70 gene sequence in poultry as the reference sequence.Total RNA was extracted from livers of heat-stressed pigeons,and the objective gene was amplified by RT-PCR.[Result] The gene fragments amplified by RT-PCR was about 576 bp,and was confirmed as HSP70 gene in pigeon by sequence measurement.The homology of HSP70 gene in pigeon was over 97%,compared with chicken,quail and guinea fowl.[Conclusion]HSP70 gene in poultry has high conservation.

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