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当前位置: 首页 > 文章 > 两株光合细菌的分离鉴定及其水质净化效果研究 淡水渔业 2013,43 (5) 57-61
Position: Home > Articles > Selection and identification of two photosynthetic bacteria strains and their purification effect for aquaculture wastewater Freshwater Fisheries 2013,43 (5) 57-61

两株光合细菌的分离鉴定及其水质净化效果研究

作  者：
刘洋;舒巧玉;楼春燕;吴湘;韩志萍;叶金云
单  位：
湖州师范学院生命科学学院
关键词：
光合细菌;分离鉴定;青鱼(Mylopharyngodon piceus);养殖污水;净化
摘  要：
为了筛选能够高效净化水质的光合细菌菌株,采用半固体试管法和双层平板法,从市售光合菌菌液中分离得到2株光合细菌,分别命名为GHJ-1、GHJ-2,对菌株进行了形态学观察、生理生化及16S rDNA鉴定,比较了这2株光合细菌对青鱼(Mylopharyngodon piceus)养殖污水的净化效果。结果表明:经鉴定这两株光合细菌为Rhodovulum sp菌株。菌株GHJ-1、GHJ-2对污水进行15 d的净化处理后,硝酸盐去除率分别为91.4%和96.3%;亚硝酸盐的去除率分别为68.9%和82.7%;氨氮去除率分别为56.8%和67.5%;COD Mn的去除率分别为61.7%和42.4%。
译  名：
Selection and identification of two photosynthetic bacteria strains and their purification effect for aquaculture wastewater
作  者：
LIU Yang;SHU Qiao-yu;LOU Chun-yan;WU Xiang;HAN Zhi-ping;YE Jin-yun;School of Life Sciences,Huzhou Normal University;
关键词：
photosynthetic bacteria(PSB);;selection and identification;;Mylopharyngodon piceus;;aquaculture wastewater;;purification
摘  要：
In order to select the photosynthetic bacteria(PSB) strains to be used for aquaculture wastewater purification,two PSB strains,named GHJ-1,GHJ-2,were isolated from commercial PSB broth by anaerobic culture,semi-solid tube and double-layer plate culture methods.The two obtained strains were investigated based on morphology,physio-biochemical characteristics and 16S rDNA sequence analysis.Their purification effects for the effluent from pond of Mylopharyngodon piceus culture were determined in the experiment.Both of the strains GHJ-1 and GHJ-2 were identified as Rhodovulum sp.The removal rates for nitrate,nitrite,ammonia nitrogen and chemical oxygen demand(COD) from the pond effluent,by strains GHJ-1 and GHJ-2,were 91.4% and 96.3%,68.9% and 82.7%,56.8% and 67.5%,61.7% and 42.4%,respectively.

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