A Simple and Convenient Method of Extracting Total RNA from Coastal Plants Clerodendron inerme and Vitex rotundifolia

YU Lin et al(College of Material Sciences and Chemical Engineering,Hainan University,Danzhou,Hainan 571737)

Coastal plants;Total RNA;Clerodendron inerme;Vitex rotundifolia

[Objective] The purpose was to introduce a simple and convenient method of extracting total RNA from leaf tissues of coastal plants.[Method] The extracts was prepared by grinding 800-1 000 mg fresh leaves of Clerodendron inerme and Vitex rotundifolia added with liquid nitrogen.The sample of total RNA was prepared by centrifugating the extracts added with reagent at 4 ℃,centrifugating at room temperature after ice bath and centrifugating at room temperature after adding reagent in order.[Result] A260/A230 of the extracted total RNA determined by spectrophotometer was bigger than 2.10,with A260/A280 of about 1.93.The RNA content in the solution was about 4.0 μg/μl.Fewer kinds of reagents were used by this method,the total RNA with better quality could be obtained by washing each column for only 4 times,and about 700 μg total RNA could be extracted by grinding 800-1 000 mg materials for once.The electrophoretic check showed that the extracted total RNA accorded the requirements of molecular biological research and could be directly used in various molecular biological experiments.[Conclusion] This method was a simple and convenient method of extracting total RNA with higher quality and output from leaf tissues of coastal plants in short time,with less agent and cost.